Endogenous levels of NAEs in brain regions of FAAH (+/+) and (−/−) mice
| Brain regions | NAE levels (pmol/gm wet weight), mean ± SEM | |||||
|---|---|---|---|---|---|---|
| Anandamide | OEA | PEA | ||||
| +/+ | −/− | +/+ | −/− | +/+ | −/− | |
| Hippocampus | 3.3 ± 0.8 | 38.0 ± 2.8* | 61.5 ± 13.6 | 1341.8 ± 123.0 | 154.5 ± 41.7 | 2149.50 ± 226.7 |
| Cortex | 1.8 ± 0.1 | 26.0 ± 2.4 | 55.4 ± 7.2 | 1094.7 ± 130.7 | 103.3 ± 27.6 | 1685.33 ± 59.3 |
| Cerebellum | 1.3 ± 0.3 | 29.7 ± 2.1 | 101.0 ± 20.1 | 1669.8 ± 161.8 | 237.2 ± 56.0 | 2401.67 ± 164.9 |
Levels for anandamide, OEA, and PEA were significantly higher in all three brain regions in FAAH (−/−) mice compared with FAAH (+/+) mice (p < 0.001; unpaired t test;n = 3–6 per group). Anandamide levels in FAAH (−/−) hippocampus were higher than in FAAH (−/−) cortex and cerebellum (
↵* p < 0.05; unpaired ttest).