Table 1.

Regulation of histone modifications at the c-fos, BDNF, and CREB promoters in the hippocampus by acute and chronic ECS

c-fosBDNF P2BDNF P3CREB
Promoter/time course Acute Chronic Acute Chronic Acute Chronic Acute Chronic
H4 acetylation
30 min 330 ± 41a −220 ± 130d 156 ± 42 −283 ± 30b
2 hr 295 ± 53a 298 ± 71a 278 ± 60a 268 ± 50a 114 ± 23 131 ± 16 490 ± 62a −125 ± 19
24 h −132 ± 32 −205 ± 49b 185 ± 30a −359 ± 79b −139 ± 51 −138 ± 14 −134 ± 32 −246 ± 37b
H3 acetylation
30 min ND ND 220 ± 98a,c −351 ± 142d
2 hr 100 ± 16 295 ± 222d −188 ± 86d 195 ± 43a −133 ± 15 −123 ± 11 −265 ± 60b −216 ± 69b,c
24 h 183 ± 67d 98 ± 29 ND 339 ± 116a,c 411 ± 54a 153 ± 69 236 ± 47a,c
H3 phosphoacetylation
30 min ND ND 344 ± 276d −239 ± 117d
2 hr 372 ± 110a 424 ± 82a 130 ± 47d 290 ± 39a 154 ± 53 −148 ± 24b 113 ± 31 103 ± 18
24 h ND ND ND ND
mRNA levels (total BDNF)
30 min 3237 ± 728a 185 ± 24a 107 ± 16
2 hr 1514 ± 418a 482 ± 44a 149 ± 19
24 h 131 ± 28 −176 ± 10b −123 ± 11 214 ± 24a −119 ± 2 −178 ± 31b
  • This is a comprehensive report of our data, expressed as percentage increase (a) or decrease (b) over control ± SEM (n = 4−6 animals in each treatment group). The values shown (a or b) represent statistically significant changes (p < 0.05 by paired t test). Percentages (c) indicate trends, changes that did not reach a level of significance (usually because of large variability in the fold increase or the fold decrease among the samples) but in which all samples showed a change in the same direction and were significant by χ2 test. The values shown (d) represent conditions for which there was large variability and hence no apparent regulation by ECS. Ct values were too high in some samples, indicating that the anti-histone antibody precipitated negligible levels of that gene and was therefore not detectable (ND).

  • a Increase in acetylation/phosphoacetylation, p < 0.05.

  • b Decrease in acetylation/phosphoacetylation, p < 0.05.

  • c Trend toward increase or decrease (−), p < 0.1.

  • d Large variability among samples, no trend for regulation.