Table 1.

Dopamine uptake characteristics for WT and hDAT mutants expressed in HEK293 cells

hDAT constructs nVmax ± SE (relative to WT) KM (μm)[SE interval]
WT 10 100% 0.42[0.36-0.48]
ΔLKV 3 5.6 ± 0.2% 0.21[0.18-0.24]
HHH 3 10.7 ± 0.6% 0.21[0.18-0.25]
β2-3 3 62.0 ± 8.1% 0.45[0.43-0.46]
β2-8 2 17.5 ± 3.0% 0.23[0.19-0.28]
β2-13 2 11.9 ± 1.8% 0.25[0.24-0.26]
β2-23 3 13.8 ± 1.8% 0.34[0.29-0.39]
β2-23(-SLL) 3 13.2 ± 2.2% 0.50[0.45-0.56]
+Ala 3 65.5 ± 12.8% 0.48[0.42-0.55]
+Tyr 3 68.8 ± 1.8% 0.44[0.43-0.46]
3×Ala_618-620 3 28.7 ± 3.3% 0.44[0.36-0.54]
3×Ala_615-617 3 6.3 ± 1.4% 0.58[0.48-0.70]
3×Ala_612-614 3 38.7 ± 4.3% 0.40[0.34-0.47]
3×Ala_609-611 3 49.0 ± 5.8% 0.40[0.32-0.50]
3×Ala_606-608 3 74.5 ± 16.9% 0.41[0.36-0.47]
3×Ala_598-600 3 73.3 ± 8.3% 0.51[0.44-0.58]
  • Vmax for WT hDAT was 1074 ± 156 fmol/min per 105 cells (mean ± SE). Each data point is normalized relative to WT hDAT from the same experiment.