Table 2.

90-10% fall time

n 90-10 time (sec)
WT 10 0.28 ± 0.03
H33A 6 0.46 ± 0.05
Q37A 5 0.06 ± 0.01*
I40A 6 1.57 ± 0.15*
L41A 6 0.07 ± 0.01*
Y43A 9 2.68 ± 0.13*
F44A 5 2.13 ± 0.24*
I50A 9 0.11 ± 0.02*
I328A 5 0.85 ± 0.19*
P329A 7 2.71 ± 0.15*
N333A 6 1.44 ± 0.19*
L338A 5 0.06 ± 0.00*
T339A 7 1.05 ± 0.09*
S340A 5 0.04 ± 0.00*
G342A 5 0.04 ± 0.00*
V343A 5 5.06 ± 0.16*
G344A 6 0.09 ± 0.02*
S345A 5 0.15 ± 0.04*
  • All drugs were applied at concentrations equal to the EC50 value of the receptor under investigation.

  • * p<0.01 for log(90 - 10 time).