Table 1.

Leaving probabilities (PL) of C. elegans strains

Genotype or condition Male Hermaphrodite
Wild type
Adult 0.17 ± 0.008 (20) 0.002 ± 0.001a* (15)
L4 <0.001b* (2) <0.001 (2)
Male with hermaphrodites <0.001c* (8) NA
Male with males 0.103 ± 0.017d* (3) NA
Male with hermaphrodites, no food 0.6 ± 0.05e* (3) NA
Gonad ablations
Entire gonad ablated 0.05 ± 0.002f* (n = 25) 0.06 ± 0.002f* (n = 23)
Germ line ablated 0.07 ± 0.003f** (n = 18) 0.005 ± 0.004f*** (n = 30)
Serotonin biosynthesis
tph-1(mg280) 0.04 ± 0.01g* (3) 0.007 ± 0.002g*** (3)
Insulin pathway
Wild type 0.05 [0.04, 0.06] (4)
daf-2(e1370) 0.003 [0.001, 0.007]g* (4)
daf-16(mgDf50) 0.11 [0.09, 0.14]g* (3) 0.003 ± 0.001***
daf-2(e1370);daf-16(mgDf50) 0.06 [0.05, 0.08]g*** (3)
Germ cell developmentg
glp-1(bn18) 0.04 ± 0.002* (2) <0.001*** (2)
spe-26(hc138) 0.05 ± 0.005* (2) 0.001 ± 0.001*** (2)
fog-1(q253) 0.001 ± 0.001* (3) 0.001 ± 0.001*** (2)
fem-1(hc17) NA 0.004 ± 0.004*** (2)
fem-2(b245) NA 0.002 ± 0.002*** (2)
fem-3(e2006) NA 0.004 ± 0.003*** (2)
fem-3(q20gf) NA 0.005 ± 0.005*** (3)
mog-3(q74) NA 0.010 ± 0.007*** (2)
  • PL ± SE was calculated from the combined data of all independent tests. For the insulin pathway male data, which were analyzed by survival analysis (see Materials and Methods), upper and lower 95% confidence intervals are given in brackets. The number of independent assays that were combined in the calculation of PL is given in parentheses. For ablation experiments, the total number of animals examined is given. p values for pairwise contrasts are indicated as follows: *p <0.001; **p <0.05; ***p >0.05. For daf-2(e1370);daf-16(mgDf50) versus daf-2(e1370), p <0.001; for daf-2(e1370);daf-16(mgDf50) versus daf-16(mgDf50), p <0.05. Insulin pathway assays were performed at 25°C. Wild-type males appear to have a significantly lower rate of leaving at this temperature than at lower temperatures, and this slow leaving apparently requires daf-16 function. A possible explanation is that the worms are experiencing some stress response at the higher temperature. NA, Not applicable.

    a-gThe data compared were as follows: awild-type hermaphrodite versus wild-type male; bL4 male versus adult male; cmale with hermaphrodites versus male alone; dmale with males versus male alone; emale with hermaphrodites without food versus male with hermaphrodites on food; fablated versus unablated; gmutant versus wild type.