Table 1.

Summary of the olfactory receptor genes expressed in the septal organ confirmed by different means

Gene name NBT—BCIP-positive cells per section (mean ± SD) Percentage in OMP-positive cells (mean ± SD) Fold-change (SO vs MOE) in genechips cDNA cloning Specific RT-PCR
Category I MOR256-3 49.8 ± 0.9%; n = 12 31.1 +
Category II MOR244-3 35.7 ± 12.1; n = 18 12.2 ± 0.8; n = 10 26.3 +
MOR0-2a 23.9 ± 8.8; n = 16 8.0 7.2 +
MOR235-1 19.4 ± 8.6; n = 17 6.5 3.2 ++++
MOR160-2 14.2 ± 4.1; n = 5 4.7 - +++
MOR122-1 10.9 ± 4.6; n = 17 3.7 - ++++
MOR160-5 8.6 ± 7.5; n = 18 2.9 2.9 +
MOR236-1 7.8 ± 3.3; n = 12 2.6 - ++
Category III MOR244-2 3.8 ± 1.8; n = 9 1.3 1.1 ++
MOR271-1 1.3 ± 2.1; n = 14 0.4 1.0 +++
MOR232-2 0.7 ± 0.8; n = 10 0.2 - +++
Category IV The following antisense RNA probes stained less than one cell per section, but their existence in the septal organ was confirmed by other means: MOR40-12 and MOR31-12 by genechips (both showed 2.6-fold increment as compared to the MOE) and specific RT-PCR; MOR13-6, MOR257-1, MOR103-15, MOR171-2, MOR279-2, MOR174-13 and MOR225-2 by cDNA cloning.
Category V The following sequences were encountered only in one RNA sample in cDNA cloning, but their existence was not verified by in situ hybridization. MOR3-1; MOR9-2; MOR13-4; MOR14-4; MOR24-2; MOR29-1; MOR30-3; MOR31-4; MOR33-1; MOR33-2; MOR103-16; MOR132-1; MOR135-9; MOR135-13; MOR135-19; MOR135-20; MOR160-1; MOR161-5; MOR163-1; MOR164-1; MOR164-3; MOR171-6; MOR171-22; MOR171-23; MOR171-24; MOR171-43; MOR171-44; MOR172-2; MOR174-10; MOR174-12; MOR174-13; MOR174-15P; MOR174-18; MOR179-3; MOR185-7; MOR185-8; MOR187-1; MOR187-2; MOR188-3; MOR194-1; MOR203-1; MOR204-1; MOR204-23; MOR206-3; MOR206-4; MOR206-6; MOR208-5P; MOR209-1; MOR211-1; MOR224-4; MOR225-6P; MOR225-8P; MOR231-11; MOR232-5; MOR239-5; MOR256-12; MOR257-2; MOR262-5; MOR264-4; MOR274-1; MOR276-1; MOR277-1; MOR281-1; MOR284-1.
  • The “NBT-BCIP positive cells” were counted and averaged (mean ± SD) from all the septal organ sections (n) stained by each RNA probe. NBT, Nitroblue tetrazolium; BCIP, 5-bromo-4-chloro-3-indolyl phosphate. The “percentage in OMP-positive cells” for MOR256-3 and MOR244-3 was obtained from double in situ hybridization, and the rest was estimated according to the MOR244-3 data generated in the two ways (see Results). The “fold-change (SO vs MOE) in genechips” was averaged from the two experiments, and a dash indicates “not detected.” The number of the plus signs under “cDNA cloning” indicates the number of RNA samples (of four) from which each sequence was encountered.

  • a MOR0-2 was previously annotated as a pseudogene MOR0-2P in GenBank (accession number AY073944). Our sequencing data indicate this gene has an intact coding region; hence we use MOR0-2 with a new accession number (AY635588).