Table 2.

Thermodynamic analysis of NKCC1 during OGD/REOX, HAIR, and HAIR/REOX

Ionic concentrations (mm)
[Na+]i [Na+]o [K+]i [K+]o [Cl]i [Cl]o ΔG
Control 12.7 137 112 5.4 36 144 −12.3
OGD/REOX 46 135 112 5.6 36 135 −8.9
OGD/REOXa 46 135 56 5.6 72 135 −8.9
HAIR 8 50 112 62 36 57 −12.5
HAIR/REOXa 52 137 56 5.4 72 144 −8.9
  • ΔG of NKCC1 under various ionic conditions was calculated using the following equation: Embedded Image.

    RT at 22°C is 2.45 kJ/mmol.

  • a [Cl]i is assumed to increase by 2×, [K+]i is assumed to decrease by 2×.