Table 2.

Effects of inhibitors for IDE on the elimination rate of [125I]Aβ(1-40) from rat brain

Inhibitor Apparent elimination rate constant (kapp,el) (percentage of control)
Control 100.0 ± 6.8
Bacitracin (1 mg/ml) 76.7 ± 8.9
N-ethylmaleimide (1 mm) 106.8 ± 5.5
Control (PBS)a 100.0 ± 7.1
1, 10-Phenanthroline (1 mm)a 99.4 ± 15.4
EDTA (5 mm)a 98.1 ± 12.8
  • Bacitracin, N-ethylmaleimide, 1, 10-phenanthroline, and EDTA were each preadministered before [125I]Aβ(1-40) and [14C]inulin administration. The clearance was evaluated as kapp,el. Each value represents the mean ± SEM (n = 3).

  • a The elimination rate was examined in the presence of 1, 10-phenanthroline or EDTA dissolved in PBS.