Influence of subtype-selective mGIu receptor ligands on [3H]thymidine incorporation in immature cultured granule cells
| [3H]thymidine incorporation (dpm/dish) × 10−3 | |||
|---|---|---|---|
| PHCCC | |||
| None | 3 μm | 30 μm | |
| Control | 366 ± 8 | 276 ± 5a | 96 ± 6a |
| MSOP (200 μm) | 457 ± 12b | 358 ± 14a,b | 244 ± 31a,b |
| MSOP (1 mm) | 422 ± 6b | ND | 233 ± 19a,b |
| MPPG (1 mm) | 375 ± 32 | 315 ± 9a,b | 194 ± 36a,b |
| MPEP (0.1 μm) | 329 ± 25 | 250 ± 14a | 88 ± 6a |
| CPCCOEt (10 μm) | 380 ± 26 | 261 ± 20a | 84 ± 12a |
| LY367385 (10 μm) | 370 ± 7 | ND | 101 ± 21a |
| LY379268 (0.1 μm) | 390 ± 8 | ND | 92 ± 15a |
| LY341495 (0.1 μm) | 360 ± 15 | 276 ± 22a | 82 ± 10a |
Cultures were exposed to drugs for 24 hr. mGlu receptor ligands were applied 10 min before PHCCC. Values are means ± SEM of six to nine determinations from two to three independent experiments. p < 0.05 (1-way ANOVA plus Fisher's PLSD) versus the respective values in the absence of PHCCC (None) (a) or the respective control values (b). ND, Not determined.