Primers for 5-HT1A promoter and genes
| DNA fragment | Primer | Primer sequence | Restriction enzyme | Encodinga |
|---|---|---|---|---|
| P1 | 5′-GCGCGGTACCTTTGAGATTAGACTGTTTTA-3′ | KpnI | (−1180)-(−1160) | |
| 5-HT1A promoter | P2 | 5′-GCGCGCGGCCGCAGACTGAACATATCCACCT-3′ | NotI | (+17)-(−4) |
| P1 | 5′-GCGCGGTACCTTTGAGATTAGACTGTTTTA-3′ | KpnI | (−1180)-(−1160) | |
| 5-HT1A promoter plus gene | S2 | 5′-GCGCAAGCTTCCCCGAAGGCTGCAAACT | HindIII | 2382-2362 |
| S | 5′-GCGCCTCGAGGCAGGCATGGATATGTTC-3′ | XhoI | (−6)-(+15) | |
| 5-HT1A sense gene | S2 | 5′-GCGCAAGCTTCCCCGAAGGCTGCAAACT | HindIII | 2382-2362 |
| S | 5′-GCGCCTCGAGGCAGGCATGGATATGTTC-3′ | XhoI | (−6)-(+15) | |
| 5-HT1A antisense | AS | 5′-GCGCGATATCGCGGCCGCATCATCAGCGAAGAACTT-3′ | EcoRV-NotI | 1273-1254 |
P1, Sense prime for 5-HT1A promoter; P2, antisense prime for 5-HT1A promoter; S, sense prime for sense sequence of 5-HT1A receptor; S2, antisense prime for sense sequence of 5-HT1A receptor; AS, antisense prime for antisense sequence of 5-HT1A receptor.
↵ a The coding ranges are according to Mus musculus serotonin 1A receptor gene, 5′ flanking sequence and partial cds (accession number U33820) and M. musculus serotonin 1A receptor mRNA, complete cds (accession number U39391, S67178). Start codon for cds = coding 1.