Table 1.

Polymorphisms within the Tas1r3 region of mouse strains with high (C57BL/6, CAST/Ei, and SWR/J) and low (129P3/J, AKR/J, and DBA/2J) saccharin preferences

PositionaAF389853b Location C57BL/6c SWR/J CAST/Ei 129P3/J DBA/J AKR/J
-2406 50853 5′ G T T T T T
-2386 50832 5′ T T T A A A
-2343 50790 5′ C DEL C DEL DEL DEL
-2342 50789 5′ A G G G G G
-2330 50777 5′ A G A G G G
-2323 50770 5′ A G G G G G
-2315 50762 5′ G DEL G DEL DEL DEL
-2198 50654 5′ DEL T T T T T
-2181 50629 5′ G T T T T T
-2002 50450 5′ C T T T T T
-1994 50442 5′ C A A A A A
-1845 50293 5′ A G A G G G
-1820 50268 5′ G A G A A A
-1803 50251 5′ G A G A A A
-1791 50239 5′ C T C T T T
-1786 50234 5′ G A A A A A
-1558 50006 5′ G UN A UN G G
-1488 49936 5′ A UN G UN G G
-1417 49865 5′ A UN G UN G G
-1414 49863 5′ DEL UN TT UN TT TT
-1407 49856 5′ C UN T UN T T
-1317 49767 5′ G UN A G G G
-1284 49734 5′ G UN G G A A
-1273 49723 5′ A A G A A A
-1260 49710 5′ C C A C C C
-1200 49650 5′ A T A T T T
-1130 49580 5′ A G A G G G
-1078 49529 5′ A G G G G G
-1032 49482 5′ T G G G G G
-967 49417 5′ C T T T T T
-962 49412 5′ T C C C C C
-912 49362 5′ C C T C C C
-827 49277 5′ AG AG DEL AG AG AG
-822 49272 5′ A A G A A A
-817 49267 5′ A G G G G G
-791 49241 5′ AGG AGG AGG DEL DEL DEL
-753 49203 5′ T T C T T T
-564 49014 5′ G C G C C C
-349 48799 5′ T C T T T T
-320 48770 5′ 12 bp DEL 12 bp DEL DEL DEL
-267 48718 5′ G DEL A DEL DEL DEL
-162 48613 5′ G C C C C C
-138 48588 5′ A A A G G G
-104 48554 5′ G G A G G G
-32 48482 5′ C T C T T T
-30 48480 5′ C T T T T T
-9 48459 5′ C A C C C C
135 48316 Exon 1 A A A G G G
163 48288 Exon 1 A A A G G G
179 48272 Exon 1 T T T C C C
182 48269 Exon 1 C T C T T T
186 48265 Exon 1 C T T T T T
264 48187 Intron DEL G G G G G
271 48180 Intron A G G G G G
272 48179 Intron G G A G G G
319 48139 Exon 2 T C T C C C
556 47902 Exon 2 A A G A A A
661 47799 Intron T T T C C C
701 47759 Exon 3 T C C C C C
974 47486 Exon 3 A G G G G G
978 47482 Exon 3 C T C C C C
1309 47151 Exon 3 G A A A A A
1466 46994 Exon 3 C T C C C C
1874 46586 Intron A A DEL A A A
1897 46563 Intron A C C C C C
2061 46399 Intron G G DEL G G G
2098 46362 Intron G G G C G G
2106 46354 Intron A A G A A A
2216 46244 Exon 6 A A G A A A
2405 46055 Exon 6 T C C C C C
2474 45985 Exon 6 G A A A A A
2531 45929 Exon 6 A A G A A A
2651 45809 Exon 6 A A T A A A
2656 45762 Exon 6 T C T T T T
2698 45762 Exon 6 T A T C C C
3145 45315 Exon 6 G A A A A A
3240 45220 3′ G G A G G G
3434 45026 3′ T C T C C C
3479 44981 3′ A A A DEL DEL DEL
3483 44977 3′ C T C T T T
3493 44967 3′ C G G G G G
3581 44870 3′ (TG)23 (TG)15 (TG)20 (TG)23 (TG)16 (TG)16
3609 44851 3′ G A G G G G
3693 44767 3′ T T C T T T
3724 44736 3′ T T C T T T
3737 44723 3′ G A G G G G
3781 44679 3′ C T C T T T
3782 44678 3′ A G A G G G
4201 44259 3′ A G A G G G
  • The 12 bp sequence at polymorphic position -320 is GCAGCTTCTTTT. The dinucleotide repeat at position +3581 is imperfect in the C57BL/6 and SWR/J strains, with G substituted with A in the second TG repeat.

    DEL, Deletion; UN, unknown. Polymorphisms significantly associated with saccharin preference are shown in bold.

  • a Polymorphism positions relative to the first nucleotide in the putative start codon of Tas1r3.

  • b Position in genomic sequence (GenBank accession number AF389853).

  • c Sequences of the C57BL/6J and C57BL/6ByJ strains were identical and shown here as C57BL/6.