Table 1.

Gene changes in neuronal versus astrocyte-enriched sorted populations

Cell type markers Fold Gene name
Neuronal markers 32.9 Distalless homeobox 1 (Dlx1)
19.2 Synaptosomal-associated protein, 25 kDa
18.6 Protocadherin α4 (Pcdha4)
14.1 Vesicular inhibitory amino acid transporter
13.4 Neuron-specific gene family member 2 (Nsg2)
11.9 Glutamic acid decarboxylase 1 (Gad1)
10.9 Protein tyrosine phosphatase, receptor type, 0
9.46 Kinesin 2, 60/70 kDa (Kns2)
9.19 Stathmin-like 3 (Stmn3)
8.88 CBFA2T1-identified gene homolog
8.71 Tubulin, β3 (Tubb3)
8.54 Neuron-specific gene family member 1 (Nsg1)
7.96 Ganglioside differentiation-associated protein 1
7.54 Distalless homeobox 5 (D1×5)
7.48 Growth-associated protein 43 (Gap43)
6.69 Distalless homeobox 2 (D1×2)
6.55 Doublecortin (Dcx)
6.29 Serpini 1
6.27 Stathmin-like 2 (Stmn2)
6.17 α-internexin
4.93 Synaptosomal-associated protein (Snap), 91 kDa
4.48 Preproenkephalin 1 (Penk1)
4.45 Neural cell adhesion molecule 1 (Ncam1)
4.39 Stathmin 1 (Stmn1)
4.29 Purkinje cell protein 4
4.27 Myelin transcription factor 1-like (Myt11)
3.70 Brain abundant signal protein 1 (Basp1)
3.28 Tubulin, β5 (Tubb5)
3.27 Profilin 2 (Pfn2)
3.01 Ca-dependent activator protein for secretion
Astrocyte markers
16.5 Aquaporin 4 (Aqp4)
16.3 Apolipoprotein E (Apoe)
15.7 Paraoxonase 2 (Pon2)
15.5 Phosphoprotein enriched in astrocytes 15
15.5 Clusterin (Clu)
11.2 Calizzarin (S100a11)
8.89 Calcyclin (S100a6)
7.54 Aldolase 3, C isoform (Aldo3)
7.53 Peripheral myelin protein, 22 kDa (Pmp2)
7.33 Histocompatibility 2, K region (H2-K)
6.54 Connexin 43
6.14 Insulin-like growth factor binding protein 5 (Igfbp5)
6.06 Cytochrome P450, 1b1 (Cyp1b1)
5.25 Annexin A2 (Anxa2)
5.17 Interleukin 10 receptor, β
4.93 N-Acylsphingosine amidohydrolase 1
4.77 Histocompatibility 2, D region locus 1
4.45 β2-microglobulin (B2m)
4.36 Cystatin C (Cst3)
4.34 Nuclear factor, erythroid-derived 2-like 2
4.09 Cd63 antigen (Cd63)
4.00 Glutathionine-S-transferase, μ1 (GSTm1)
3.96 Acid sphingomyelinase-like 3a (Asm13)
3.59 Follistatin-like (Fst1)
3.52 Phospholipase A2, group VII (Pla2g7)
3.50 Lectin, galactose binding, soluble 1
3.31 Leucine-rich repeat interacting protein 1
3.21 Glial fibrillary acidic protein (GFAP)
3.16 Quaking (qk)
3.06 1-Cys peroxiredoxin (aop2)
  • Fold, Fold change of the vehicle-treated astrocyte-enriched cell population (GFP-positive sort) compared with the neuronal cell population (GFP-negative sort). n = 3 (two comparisons of sorted, 50 MOI ad-GFP-infected cultures confirmed by one comparison of sorted populations from cultures derived from GFAP-driven GFP mice).