PCR conditions for XT-1, GAPDH, and cyclophilin
| Name | Sequence | Length of end product | Reference |
|---|---|---|---|
| XT-1 | XT1s1 | 316bp | Goetting et al. (2000) |
| 5′-CTTCATCCGCCTGGGTCTTCG-3′ | |||
| XT1as1 | |||
| 5′-GTAGTGTGTGAATTCCGCAGTGG-3′ | |||
| nested XT1s2 | |||
| 5′-GGGCTGAGTCATCGCTACACACG-3′ | |||
| nested XT1as2 | |||
| 5′-CGATGACATTGACAGGATCCACC-3′ | |||
| GAPDH | GAPDHs | 78bp | Tajima et al. (1999) |
| 5′-GAACATCATCCCTGCATACA-3′ | |||
| GAPDHas | |||
| 5′-CCAGTGAGCTTCCCGTTCA-3′ | |||
| Cyclophilin | cyclo as | 65 bp | Danielson et al. (1988) |
| 5′-TGTGCCAGGGTGGTGACTT-3′ | |||
| cyclo as | |||
| 5′-TTTCTCTCCGTAGATGGACTT-3′ |