Agonist response parameters obtained in two-electrode voltage-clamp experiments.
| Fitted parameter | α1 GlyRs | α1(R271K) GlyRs | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Glycine (n = 8) | β-Alanine (n = 5) | Taurine (n = 3) | Glycine (n = 5) | β-Alanine (n = 5) | ||||||
| −PRO | +PRO | −PRO | +PRO | −PRO | +PRO | −PRO | +PRO | −PRO | +PRO | |
| EC50 (mm) | 0.39 ± 0.05 | 0.037 ± 0.008* | 2.02 ± 0.21 | 0.09 ± 0.02* | 4.51 ± 1.96 | 0.14 ± 0.01 | 2.05 ± 0.07 | 0.18 ± 0.05* | 5.77 ± 1.38 | 0.57 ± 0.16* |
| EC50 ratio | 10.5 | 22.5 | 32.0 | 11.6 | 10.2 | |||||
| ϵ | 0.95 ± 0.01 | 0.93 ± 0.02 | 0.67 ± 0.05 | 0.92 ± 0.02* | 0.23 ± 0.04 | 0.90 ± 0.04* | 0.96 ± 0.01 | 4.1 ± 0.46* | 0.12 ± 0.05 | 1.9 ± 0.47* |
| ϵ ratio | 1.0 | 1.4 | 3.9 | 4.3 | 15.8 | |||||
EC50 and ϵ values were averaged from curve fits of individual experiments and are expressed as means ± SEM. *Different by p < 0.05 from the agonist-alone condition. +PRO, Responses recorded after 2 min of preincubation with 0.5 mm PRO.