Table 1.

Comparison of burst parameters in wild-type and Nav1.6−/− mutant bursts

Nav1.6+/+ (n = 19) Nav1.6−/− (n = 16) t test results
Action potential peak +41 ± 3 mV +32 ± 7 mV p < 0.001**
Interspike voltage −68 ± 4 mV −64 ± 4 mV p = 0.01*
Interspike interval 6.4 ± 3.2 ms 8.4 ± 2.8 ms p = 0.07
Action potential threshold −53 ± 3 mV −43 ± 3 mV p < 0.002**
Action potential width (at 0 mV) 0.41 ± 0.07 ms 0.43 ± 0.06 ms p = 0.589
Afterhyperpolarization −74 ± 2 mV −70 ± 4 mV p = 0.003*
Afterdepolarization −68 ± 3 mV −62 ± 5 mV p < 0.001**

  • The action potential peak was measured for the first spike of the burst. The interspike voltage was averaged over a 1.3 ms time window starting 1.5 ms after the peak of the first action potential. The interspike interval was measured between the peaks of the first and second spikes. The action potential threshold was defined as the voltage at which dV/dt exceeded 20 mV/ms (measured for the first action potential), and action potential width was defined as the width at 0 mV. The afterhyperpolarization was the most negative voltage reached after the first spike, and the afterdepolarization was the most positive voltage reached after the last spike. *p ≤ 0.01; **p < 0.002.