Table 1.

Blockers of TRPA1 channels in HEK activated by AITC and of the endogenous mechanotransducer channel of hair cells activated mechanically

Blocker TRPA1 in HEKs Hair cell transducer References
Gentamicin-IC50 6.7 ± 0.68 μm 7.6 μm (Kroese et al., 1989)
Gentamicin-Hill coefficient 1.16 1.18 (Kroese et al., 1989)
Ruthenium red-IC50 3.44 ± 0.09 μm 3.6 ± 0.3 μm (Farris et al., 2004)
Ruthenium red-Hill coefficient 1.13 ± 0.03 1.4 ± 0.2 (Farris et al., 2004)
Gd3+-IC50 0.10 ± 0.02 μm 10.1 μm (Kimitsuki et al., 1996)
Gd3+-Hill coefficient 1.17 ± 0.25 1.14 (Kimitsuki et al., 1996)
Amiloride-IC50 511 ± 11.7 μm 50 μm (Jorgensen and Ohmori, 1988; Rüsch et al., 1994)
Amiloride-Hill coefficient 2.43 ± 0.12 2.2 ± 0.1 (Ricci, 2002)
  • Note that although blocking effects on TRPA1 were tested in the absence of external calcium to avoid calcium-induced potentiation and inactivation, the blocking effects on hair cells were tested in the presence of external calcium, because in its absence, the tip links disassemble and mechanotransduction is abolished. Data for TRPA1 show mean ± confidence interval.