Indices of Iocomotor activity of male and female HAB-M, NAB-M, CM-M, and LAB-M mice in the EPM, the dark-light avoidance test, and the home cage
| HAB-M | NAB-M | CM-M | LAB-M | |
|---|---|---|---|---|
| Males | ||||
| Elevated plus-maze test | n = 21 | n = 36 | n = 46 | n = 12 |
| Closed-arm entries (two paws) | 11.1 ± 0.9 | 12.8 ± 0.6### | 15.4 ± 0.6**,### | 6.9 ± 1.0*** |
| Open-arm entries (two paws) | 1.0 ± 0.4 | 5.7 ± 0.4***,### | 5.8 ± 0.5***,### | 8.9 ± 0.9*** |
| Open-arm entries (four paws) | 0.2 ± 0.2 | 3.3 ± 0.4***,### | 2.3 ± 0.4***,### | 8.6 ± 1.0*** |
| Ratio open/total entries (%) | 6.0 ± 1.9 | 30.6 ± 1.9***,### | 25.5 ± 1.6***,### | 56.6 ± 4.8*** |
| Dark-light avoidance test | n = 23 | n = 12 | n = 28 | |
| Transitions | 11.3 ± 1.8 | 19.8 ± 2.5**,### | 10.0 ± 1.1 | |
| Line crossings total | 92.9 ± 7.7 | 153 ± 13.5*,### | 265 ± 17.4*** | |
| Home cage activity (8:00 A.M. to 12:00 A.M.) | n = 4 | n = 4 | n = 5 | |
| Locomotion (%) | 0.7 ± 0.5 | 1.6 ± 0.6# | 8.5 ± 1.5* | |
| Females | ||||
| Elevated plus-maze test | n = 30 | n = 15 | n = 49 | n = 12 |
| Closed-arm entries (two paws) | 12.3 ± 1.2 | 15.1 ± 1.4### | 13.3 ± 0.4### | 7.0 ± 1.0*** |
| Open-arm entries (two paws) | 1.2 ± 0.3 | 6.1 ± 0.7***,# | 6.3 ± 0.5***,# | 8.4 ± 0.6*** |
| Open-arm entries (four paws) | 0.2 ± 0.1 | 2.9 ± 0.6***,### | 3.9 ± 0.4***,### | 7.9 ± 0.7*** |
| Ratio open/total entries (%) | 7.6 ± 1.5 | 28.4 ± 1.5***,### | 30.4 ± 1.7***,### | 55.8 ± 3.9*** |
| Dark-light avoidance test | n = 28 | n = 12 | n = 30 | |
| Transitions | 7.6 ± 1.2 | 23.9 ± 3.2***,### | 14.6 ± 1.5** | |
| Line crossings total | 74.6 ± 7.2 | 172 ± 10.5***,### | 288 ± 12.6** | |
| Home cage activity (8:00 A.M. to 12:00 P.M.) | n = 4 | n = 4 | n = 4 | |
| Locomotion (%) | 1.5 ± 0.6 | 2.3 ± 1.0# | 5.7 ± 1.4** |
Two-paw and four-paw entries into the open arms of the EPM are highly correlated (r 0.85; p < 0.001; n = 221). The number of animals are given for each line and test. Home-cage activity was scored during the regular testing time (8:00 A.M. to 12:00 P.M.), with n indicating the number of cages watched. *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding HAB-M; #p < 0.05, ##p < 0.01, ###p < 0.001 versus corresponding LAB-M.