Statistical summary of the effects of APα on cell-cycle gene expression using cDNA array analysis
| Gene in categories | Multivariate statistic (APα vs control) | Univariate analysis (APα vs control) | Direction of change (APα vs control) |
|---|---|---|---|
| Cyclins | p < 0.05 | Cyclin B, p < 0.05 | Increased |
| Cyclin E2, p < 0.05 | |||
| CDKs | p < 0.05 | Cdc2 (CDK1), p < 0.05 | Increased |
| CDK inhibitors | p < 0.05 | CDK int 16, p < 0.05 | Decreased |
| Ubiquitin relatives | p < 0.05 | Cullin 3, p < 0.05 | Decreased |
| Ube1X, p < 0.05 | |||
| Cullin 1, p < 0.05 | |||
| PCNA | p < 0.05 | PCNA, p < 0.05 | Increased |
| Actin and GAPDH | p < 0.5 | GAPDH, p < 0.5 | No change |
Cultures of hippocampal neurons were treated with vehicle or 500 nm APα for 24 h, followed by extraction of total RNA. Nonradioactive probes were prepared and hybridized to gene array. Data were determined by optical density and subjected to multivariate ANOVA. Ube1X, Ubiquitin-activating enzyme E1. CDK int 16, CDK inhibitor P16.