4 months of age | 6 months of age | |||||
---|---|---|---|---|---|---|
Region | WT (6) | Fmr1 null (8) | WT (6) | Fmr1 null (6) | F(1,22) age | F(1,22) genotype |
Cortex | ||||||
Frontal association | 4.2 ± 0.3 | 4.8 ± 0.2 | 3.4 ± 0.2 | 4.0 ± 0.4 | 11.38** | 4.67* |
Prelimbic | 5.2 ± 0.3 | 5.7 ± 0.2 | 4.3 ± 0.3 | 4.7 ± 0.4 | 11.14** | 1.79 |
Piriform | 7.0 ± 0.4 | 7.7 ± 0.3 | 5.6 ± 0.3 | 6.1 ± 0.5 | 16.58*** | 2.52 |
Primary motor | 4.8 ± 0.3 | 5.2 ± 0.2 | 3.8 ± 0.2 | 4.3 ± 0.4 | 11.53** | 3.07 |
Primary somatosensory | 5.2 ± 0.3 | 5.6 ± 0.2 | 4.2 ± 0.2 | 4.7 ± 0.4 | 10.86** | 1.80 |
Barrel | 5.4 ± 0.3 | 5.9 ± 0.2 | 4.3 ± 0.3 | 4.6 ± 0.4 | 18.38*** | 2.01 |
Cingulate | 5.7 ± 0.4 | 6.5 ± 0.2 | 4.8 ± 0.2 | 5.0 ± 0.4 | 13.45** | 2.89 |
Posterior parietal | 5.0 ± 0.3 | 5.9 ± 0.2† | 4.0 ± 0.2 | 4.7 ± 0.3 | 18.23*** | 8.40** |
Auditory | 5.3 ± 0.4 | 5.7 ± 0.2 | 4.0 ± 0.3 | 4.3 ± 0.3 | 23.09*** | 1.81 |
Visual | 5.4 ± 0.4 | 5.8 ± 0.2 | 4.0 ± 0.3 | 4.4 ± 0.4 | 22.14*** | 2.02 |
Hippocampal formation | ||||||
Hippocampus | ||||||
Stratum oriens | 3.1 ± 0.2 | 3.7 ± 0.2 | 2.5 ± 0.2 | 3.2 ± 0.4 | 5.06* | 6.22* |
Pyramidal cell layer, CA1 | 6.7 ± 0.4 | 7.6 ± 0.2 | 5.0 ± 0.3 | 6.2 ± 0.4† | 22.39*** | 9.95** |
Pyramidal cell layer, CA2 | 10.2 ± 0.5 | 11.2 ± 0.3 | 7.4 ± 0.6 | 8.6 ± 0.6 | 33.01*** | 5.42* |
Pyramidal cell layer, CA3 | 10.1 ± 0.6 | 11.1 ± 0.2 | 7.7 ± 0.3 | 8.9 ± 0.7 | 23.31*** | 5.57* |
Stratum lucidem | 4.9 ± 0.2 | 5.2 ± 0.2 | 4.0 ± 0.2 | 4.3 ± 0.3 | 16.90*** | 2.40 |
Stratum radiatum | 2.6 ± 0.2 | 2.9 ± 0.2 | 1.9 ± 0.2 | 2.3 ± 0.1 | 14.16** | 3.49 |
Stratum lacunosum moleculare | 2.2 ± 0.2 | 2.5 ± 0.2 | 1.6 ± 0.1 | 1.9 ± 0.1 | 12.17** | 3.46 |
Dentate gyrus | ||||||
Molecular layer | 2.6 ± 0.2 | 3.0 ± 0.1 | 1.9 ± 0.2 | 2.3 ± 0.1 | 18.66*** | 4.47* |
Granular layer | 7.6 ± 0.6 | 8.1 ± 0.2 | 5.9 ± 0.4 | 6.6 ± 0.5 | 14.20** | 2.56 |
Polymorph layer | 5.2 ± 0.5 | 5.7 ± 0.2 | 4.1 ± 0.2 | 4.6 ± 0.2 | 13.22** | 2.59 |
Presubiculum | 5.7 ± 0.3 | 6.4 ± 0.3 | 3.5 ± 0.8 | 5.0 ± 0.4 | 14.89*** | 5.40* |
Subiculum | 5.7 ± 0.3 | 6.2 ± 0.3 | 4.2 ± 0.3 | 4.9 ± 0.4 | 19.53*** | 3.31 |
Subcortical areas | ||||||
Basolateral amygdala | 5.7 ± 0.4 | 6.4 ± 0.2 | 4.5 ± 0.2 | 5.2 ± 0.4 | 18.97*** | 5.62* |
Bed nucleus of the stria terminalis | 3.3 ± 0.2 | 3.6 ± 0.1 | 2.6 ± 0.1 | 2.8 ± 0.2 | 16.77*** | 2.17 |
Nucleus accumbens | 3.4 ± 0.2 | 3.7 ± 0.1 | 2.8 ± 0.1 | 3.0 ± 0.3 | 10.59** | 2.04 |
Medial septal nucleus | 5.2 ± 0.4 | 5.7 ± 0.2 | 4.4 ± 0.2 | 4.9 ± 0.4 | 7.12* | 2.69 |
Lateral septal nucleus | 4.1 ± 0.3 | 4.4 ± 0.1 | 3.5 ± 0.1 | 3.9 ± 0.4 | 5.52* | 2.08 |
Values are the means ± SEM for the number of mice indicated in parentheses. Results were analyzed by means of two-way ANOVA (age by genotype). There were no statistically significant interactions.
↵* p < 0.05; statistically significant main effect.
↵** p < 0.01; statistically significant main effect.
↵*** p < 0.001; statistically significant main effect.
↵† p < 0.05; statistically significantly different from age-matched WT; post hoc t test.