Effects of null mutation for Fmr1 on rCPS (nmol · g−1 · min−1) in regions of white matter, cerebellum, pons, and medulla
| 4 months of age | 6 months of age | |||||
|---|---|---|---|---|---|---|
| Region | WT (6) | Fmr1 null (8) | WT (6) | Fmr1 null (6) | F(1,22) age | F(1,22) genotype |
| White matter | ||||||
| Anterior commissure | 2.1 ± 0.1 | 2.4 ± 0.1 | 1.7 ± 0.1 | 1.8 ± 0.2 | 19.02*** | 3.72 |
| Corpus callosum | 2.1 ± 0.1 | 2.3 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.2 | 22.70*** | 2.17 |
| Cingulum | 2.5 ± 0.1 | 2.8 ± 0.1 | 2.0 ± 0.1 | 2.1 ± 0.2 | 20.03*** | 2.74 |
| Hippocampal fimbria | 2.0 ± 0.2 | 2.4 ± 0.2 | 1.5 ± 0.1 | 1.7 ± 0.1 | 16.03*** | 5.47* |
| Optic chiasm | 1.5 ± 0.2 | 1.9 ± 0.2 | 1.2 ± 0.1 | 1.3 ± 0.1 | 10.14** | 3.16 |
| Optic tract | 1.6 ± 0.2 | 2.1 ± 0.2 | 1.4 ± 0.1 | 1.5 ± 0.1 | 7.76* | 4.12 |
| Fornix | 2.4 ± 0.1 | 2.6 ± 0.1 | 2.2 ± 0.2 | 2.3 ± 0.2 | 4.31* | 2.05 |
| Cerebellum | ||||||
| Vermis 6 | 6.7 ± 0.4 | 7.0 ± 0.2 | 5.1 ± 0.3 | 6.2 ± 0.8 | 7.59* | 2.76 |
| Vermis 7 | 6.4 ± 0.4 | 6.7 ± 0.2 | 4.7 ± 0.3 | 5.6 ± 0.6 | 14.08** | 2.90 |
| Vermis 8 | 6.4 ± 0.4 | 6.5 ± 0.2 | 4.6 ± 0.4 | 5.6 ± 0.5 | 14.08** | 2.12 |
| Vermis 9 | 7.0 ± 0.4 | 7.5 ± 0.2 | 5.3 ± 0.3 | 6.1 ± 0.6 | 17.10*** | 3.24 |
| Whole cerebellum | 6.8 ± 0.4 | 7.2 ± 0.2 | 4.8 ± 0.4 | 5.6 ± 0.5 | 24.88*** | 2.62 |
| Pons and medulla | ||||||
| Dorsal raphe | 8.3 ± 0.6 | 8.7 ± 0.3 | 5.4 ± 0.5 | 7.1 ± 0.8 | 16.33*** | 3.76 |
| Median raphe | 7.3 ± 0.5 | 8.2 ± 0.4 | 5.2 ± 0.5 | 6.4 ± 0.6 | 16.79*** | 5.21* |
| Periaqueductal grey | 4.6 ± 0.2 | 4.8 ± 0.2 | 3.2 ± 0.3 | 3.8 ± 0.4 | 18.16*** | 2.09 |
| Ventral tegmental nucleus | 4.2 ± 0.3 | 4.8 ± 0.2 | 3.2 ± 0.2 | 3.6 ± 0.4 | 16.07*** | 3.10 |
| Pontine nucleus | 7.4 ± 0.4 | 7.9 ± 0.3 | 5.3 ± 0.5 | 6.1 ± 0.6 | 20.40*** | 2.56 |
| Locus ceruleus | 9.3 ± 0.7 | 9.2 ± 0.3 | 6.2 ± 0.4 | 8.5 ± 0.8† | 12.13** | 4.01 |
| Nucleus of the solitary tract | 5.9 ± 0.4 | 6.1 ± 0.2 | 4.2 ± 0.3 | 4.4 ± 1.0 | 10.55** | 0.21 |
| Dorsal motor nucleus of vagus | 8.0 ± 0.4 | 8.8 ± 0.3 | 6.2 ± 0.5 | 7.9 ± 0.6† | 9.71** | 7.59* |
| Hypoglossal nucleus | 7.2 ± 0.5 | 7.7 ± 0.2 | 5.3 ± 0.4 | 6.2 ± 0.5 | 17.36*** | 3.12 |
Values are the means ± SEM for the number of mice indicated in parentheses. Results were analyzed by means of two-way ANOVA (age by genotype). There were no statistically significant interactions.
↵* p < 0.05; statistically significant main effect.
↵** p < 0.01; statistically significant main effect.
↵*** p < 0.001; statistically significant main effect.
↵† p < 0.05; statistically significantly different from age-matched WT; post hoc t test.
†† p < 0.01; statistically significantly different from age-matched WT; post hoc t test.