Proenkephalin-A mRNA expression in the pPVN and perifornical region in virgin and pregnant (day 21) rats
| pPVN | Perifornical region | |||
|---|---|---|---|---|
| Group | Number of positive cells | Grain density (arbitrary units) | Number of positive cells | Grain density (arbitrary units) |
| Virgin | 36.1 ± 6.5 (n=10) | 0.249 ± 0.02 (n=8) | 29.1 ± 3.2 (n= 9) | 0.317 ± 0.03 (n=8) |
| Pregnant (day 21) | 40.9 ± 3.2 (n=11) | 0.234 ± 0.01 (n=8) | 31.9 ± 3.6 (n=11) | 0.310 ± 0.03 (n=8) |
Coronal brain sections (15 μm) were hybridized with an 35S-labeled oligonucleotide probe complementary to pENK-A mRNA. Values are the mean (±SEM) number of cells in the pPVN and perifornical region expressing pENK-A mRNA and mean (±SEM) grain density over these regions.