Table 1.

V1/2 values in absence or presence of retigabine for point mutations within the N-terminal part of S5 of KCNQ3(TD;Q1), KCNQ2-5 wild-type, and KCNQ1, KCNQ2, KCNQ4, and KCNQ5 trytophan mutants

Mutation V1/2 (mV) V1/2 (mV; 10 μm RGB) V1/2 (mV; 100 μm RGB) ΔV1/2 (mV; 10 μm RGB) ΔV1/2 (mV; 100 μm RGB)
Q3-A253V −13.3 ± 1.1 −48.4 ± 1.1 −35.1
Q3-I254V −38.2 ± 0.7 −63.4 ± 0.7 −25.2
Q3-C255F −41.7 ± 0.7 −78.4 ± 1.2 −36.7
Q3-A256I −13.1 ± 1.3 −38.6 ± 1.3 −25.5
Q3-S258R −56.5 ± 1.1 −97.4 ± 0.3 −40.9
Q3-K259Q 2.7 ± 1.4 −31.3 ± 1.1 −34.0
Q3-A264T −0.5 ± 0.4 −31.5 ± 1.1 −31.0
Q3-W265L −73.6 ± 0.6 −73.3 ± 0.4 −73.1 ± 0.4 0.3 0.5
Q1 wild-type −29.1 ± 1.5 −30.9 ± 2.4 −32.6 ± 2.5 −1.8 −3,5
Q2 wild-type −38.8 ± 0.8 −55.9 ± 0.6 −75.5 ± 0.7 −17.1 −36.7
Q3 wild-type −50.0 ± 0.9 −89.4 ± 1.9 −93.7 ± 4.9 −39.4 −43.7
Q4 wild-type −11.7 ± 1.1 −16.3 ± 1.5 −36.5 ± 1.2 −4.6 −24.8
Q5 wild-type −40.6 ± 0.8 −45.7 ± 1.3 −60.6 ± 1.2 −5.1 −20.0
Q2-W236L −52.7 ± 0.6 −53.8 ± 0.7 −54.4 ± 0.7 −1.1 −1.7
Q4-W242L −15.9 ± 1.8 −14.6 ± 1.9 −14.7 ± 1.8 +1.3 +1.2
Q5-W235L −43.6 ± 1.3 −42.7 ± 1.4 −45.3 ± 1.3 0.9 −1.7
Q1-L266W −45.3 ± 0.8 −49.4 ± 0.9 −53.2 ± 1.4 −4.1 −7.9
  • RGB, Retigabine.