Expression of Trk and Pincher affects endosomal processing of multivesicular bodies
| Viral infection(s) | MVB/cell* | Diameter (μm) | Partial (%) | Filled (%) | e− dense (%) | Int. (% gold) | Ext. (% gold) |
|---|---|---|---|---|---|---|---|
| GFP (control) | 1.42 ± 0.96(31)a | 0.24 ± 0.07(44)b | 93 | 7 | 0 | ||
| TrkB-GFP | 3.00 ± 1.18(14)a | 0.27 ± 0.10(42)b | 3 | 52 | 45 | 88 (256/40)c | 12 (34/40)c |
| TrkB-GFP plus Pincher | 13.25 ± 11.09(4)a | 0.27 ± 0.08(53)b | 92 | 6 | 2 | ND | ND |
| Pincher | 4.29 ± 2.69(7)a | 0.49 ± 0.16(30)b** | 57 | 43 | 0 | 23 (19/30)c | 77 (62/30)c |
The table presents data obtained from EM photomicrographs of soma from cultured SCG neurons that were single or double infected with the indicated adenoviruses as described in Materials and Methods. MVBs were scored for the characteristics indicated, as to mean number per cell (MVB/cell), mean diameter, partially filled (Partial), or completely filled (Filled) with vesicles, the proportion that was lysosomally dark or electron dense (e− dense), and the portion of gold particles found on the internal vesicles (Int.) or on the external MVB membrane (Ext.). Comparisons were statistically evaluated using the Welch-Satterthwaite t test. ND, Not determined.
↵ a n value for cells.
↵ b n value for endosomes.
↵ c Number of gold particles/number of endosomes.
↵* Comparisons of values to GFP control were significant to p < 0.0001.
↵** Comparison to GFP control, p < 0.0001.