Resolution of laser-scanning confocal microscope used in this study
| Fluorophore | Resolution (μm) | FWHM (μm) | ||
|---|---|---|---|---|
| λex/λem(nm) | Lateral | Axial | Lateral | Axial |
| TetraSpeck | 0.19 | 0.46 | 0.22 | 0.45 |
| 488/515 | ||||
| TetraSpeck | 0.21 | 0.51 | 0.25 | 0.64 |
| 543/580 | ||||
| TetraSpeck | 0.25 | 0.60 | 0.27 | 0.73 |
| 633/680 | ||||
| Alexa Fluor 488 | 0.19 | 0.46 | Not measured | |
| 488/519 | ||||
| Rhodamine (micro-ruby) | 0.21 | 0.51 | Not measured | |
| 543/580 | ||||
| Alexa Fluor 647 | 0.24 | 0.60 | Not measured | |
| 633/668 | ||||
Resolution was calculated according to the following, which describe theoretical limits for confocal microscopy: lateral = (0.51 × λem)/NA, and axial = (0.88 × λex)/[n - (n2 - NA2)]½, where λem is the peak emission wavelength, λex is the laser excitation wavelength, n is the refractive index of medium, and NA is the numerical aperture of the microscope objective. FWHM, Full width at half-maximum.