Table 1.

Concentration and combinations of the primary antibodies used in the double-labeling immunofluorescence experiments

Primary antisera Primary antisera
Rabbit anti-CB1 receptor (1:500) This study Mouse anti-CCK (1:2000) CURE: Digestive Diseases Research Center, University of California, Los Angeles, CA
Rabbit anti-CB1 receptor (1:500) This study Mouse anti-PV (1:1000) Sigma
Rabbit anti-CB1 receptor (1:500) This study Mouse anti-VIP (1:10000) Biogenesis, Kingston, NH
Rabbit anti-CB1 receptor (1:500) This study Mouse anti-CaBP (1:500) Swant, Bellinzona, Switzerland
Rabbit anti-CB1 receptor (1:500) This study Mouse anti-CR (1:5000) Swant
Rabbit anti-CB1 receptor (1:500) This study Rat anti-SOM (1:200) Chemicon, Temecula, CA
Rabbit anti-CaBP (1:500) Baimbridge et al., 1982 Mouse anti-CCK (1:2000) CURE: Digestive Diseases Research Center
Rabbit anti-VIP (1:2000) Gulyas et al., 1990 Mouse anti-CCK (1:2000) CURE: Digestive Diseases Research Center
Rabbit anti-CaBP (1:500) Baimbridge et al., 1982 Rat anti-SOM (1:200) Chemicon
  • The antibody concentrations used in this experiment, the combinations that were investigated, and the reference for the primary antibodies are shown. The specificity of the rabbit anti-CB1 in the cortex was confirmed in this study in CB1 knock-out animals. The specificity of the other antisera was confirmed by the laboratory or company of origin.