Activation of the Reelin signaling pathway enhances the response of cortical neurons to glutamate
| Treatment | Percentage of responsive cells | n (number of coverslips) |
|---|---|---|
| Group 1 | ||
| Control | 29.7 ± 1.6 | 5 |
| Reelin | 57.5 ± 4.4** | 5 |
| Reelin plus Ca2+-free ACSF | 2.50 ± 2.5 | 4 |
| Reelin plus d-APV | 5.50 ± 3.2 | 4 |
| Group 2 | ||
| Mock control | 10.4 ± 7.4 | 5 |
| GST plus Reelin | 44.6 ± 11.2* | 5 |
| GST—RAP plus Reelin | 15.3 ± 1.7 | 4 |
| Group 3 | ||
| Dab1 +/+, control | 24.6 ± 8.8 | 4 |
| Dab1 +/+, Reelin | 47.9 ± 6.6** | 7 |
| Dab1 -/-, control | 14.4 ± 4.5 | 10 |
| Dab1 -/-, Reelin | 11.7 ± 3.4 | 9 |
| Group 4 | ||
| PP2, control | 5.57 ± 3.8 | 7 |
| PP2, Reelin | 10.6 ± 3.3 | 5 |
| PP3, control | 27.4 ± 8.8 | 7 |
| PP3, Reelin | 48.1 ± 18.3* | 5 |
At 12-13 DIV, the cultured cortical neurons were subjected to different treatments and stimulated with glutamate in the presence of TTX, nimodipine, and CNQX. Percentage of responsive cells of each individual coverslip was calculated as described in Materials and Methods. Data are presented in the form of mean ± SE. *p < 0.05 and **p < 0.01 versus the first control in each group, one-way ANOVA test. The data in this table reflect the results of individual coverslips from all experiments examined over the course of this study to test for overall statistical significance. Figures 2, 3, 4, 5 reflect results from individual and internally controlled experimental sessions.