Passive and active membrane properties of EGFP-, Kv4.2DN plus EGFP-, and Kv2.1DN plus EGFP-expressing cortical neurons a
| Cm | Rin | RMP (mV) | APthresh (pA) | APA (mV) | APD50 (ms) | APD90 (ms) | AHP (mV) | |
|---|---|---|---|---|---|---|---|---|
| EGFP (n = 30) | 23 ± 2 | 1.1 ± 0.2 | −55 ± 1 | 40 ± 5 | 104 ± 3 | 3.8 ± 0.2 | 6.7 ± 0.4 | −7.8 ± 0.4 |
| Kv4.2DN plus EGFP (n = 25) | 22 ± 2 | 1.6 ± 0.3* | −56 ± 4 | 20 ± 5** | 96 ± 6 | 8.4 ± 0.7** | 17.1 ± 1.3** | −1.9 ± 0.3** |
| Kv2.1DN plus EGFP (n = 10) | 23 ± 2 | 1.2 ± 0.3 | −55 ± 2 | 39 ± 6 | 99 ± 6 | 3.8 ± 0.2 | 13.0 ± 0.7* | −3.4 ± 1.7* |
RMP, Resting membrane potential; AHP, afterhyperpolarization; APthresh, current threshold to evolve a single action potential in response to a 5 ms depolarizing input; APA, action potential amplitude; ADP50 and ADP90, action potential durations at 50 and 90% repolarization.
aAll values are means ± SEM. Values are significantly different (*p < 0.05; **p < 0.01) from those determined in cells expressing EGFP alone.