The effect of 3 μm etomidate on the properties of mIPSCs and the tonic current of VB neurons of WT, β2N265S, and β2–/– mice
| Control | +3 μm Etomidate | |||||
|---|---|---|---|---|---|---|
| VB neurons | WT | β2N265S | β2-/- | WT | β2N265S | β2-/- |
| mIPSC amplitude (pA) | 69 ± 5 | 94 ± 8 | 20 ± 2 | 73 ± 7 | 79 ± 10 | 22 ± 1.9 |
| mIPSC τW (ms) | 5 ± 0.8 | 3.5 ± 0.1 | ND | 14.4 ± 2.3* | 5.9 ± 0.4* | ND |
| mIPSC charge (fC) | 357 ± 30 | 435 ± 70 | 66 ± 11 | 834 ± 101* | 531 ± 42* | 82 ± 6 |
| Phasic charge (fC/s) | 18,779 | 22,881 | 1373 | 43,869 | 27,931 | 1706 |
| Tonic current (pA) | 62 ± 12 | 70 ± 13 | 28 ± 2 | +152 ± 15 | +60 ± 7 | +42 ± 6 |
| Tonic charge (fC/s) | 62,000 | 70,000 | 28,000 | +152,000 | +60,000 | +42,000 |
| Total charge (fC/s) | 80,779 | 92,881 | 29,373 | +177,090 | +65,050 | +42,333 |
The effect of etomidate on the tonic current is expressed as the change in the holding current (i.e., the current induced) by the anesthetic. Total inhibitory charge induced by etomidate is calculated as the sum of the etomidate-induced change in the phasic charge and the etomidate-induced increase in the tonic current.*p < 0.01 versus control (paired Student's t test; n = 4-6 neurons). ND, Not determined