Table 1.

Ultrastructural characteristics of axosomatic boutons and symmetric synapses

OVX+O (n = 4) OVX+E (n = 4) p values Combined (n = 8)
Bouton volume (all boutons; μm3) 0.37 ± 0.04 0.31 ± 0.03 0.23 0.34 ± 0.02
Bouton volume (with mitochondria; μm3) 0.45 ± 0.05 0.38 ± 0.04 0.25 0.41 ± 0.03
Bouton volume (lacking mitochondria; μm3) 0.12 ± 0.02 0.11 ± 0.04 0.73 0.12 ± 0.02
Vesicle density (#/μm3) 1654 ± 139 1301 ± 198 0.20 1477 ± 130
Boutons with mitochondria (%) 74 ± 1 78 ± 5 0.49 76 ± 2
Presynaptic density area (μm2) 0.10 ± 0.02 0.07 ± 0.01 0.20 0.08 ± 0.01
Multiple synapse boutons (%) 34 ± 4 36 ± 5 0.72 35 ± 1
  • Data are mean ± SEM. No significant differences between OVX+O and OVX+E were observed, so data are also presented for both groups combined.