Table 2.

Summary of the points of no net-flux (y = 0; estimate of extracellular glutamate content) and the slopes of the linear regression analyses (estimate of glutamate clearance) derived from the no net-flux microdialysis study in WT and KO mice infused intra-PFC with AAVs carrying GFP, Homer1a, or Homer1c

AAV Genotype (n) y = 0 (μm) Slope Glutamate (pmol)
GFP WT (6) 2.92 ± 0.48 1.06 ± 0.08 82.4 ± 16.3
KO (9) 10.81 ± 2.61* 1.43 ± 0.38 135.8 ± 14.8*
Homer1a WT (9) 4.16 ± 1.05 1.19 ± 0.11 94.0 ± 17.1
KO (9) 9.55 ± 2.51* 1.28 ± 0.17 154.2 ± 19.6*
Homer1c WT (6) 4.84 ± 1.40 1.47 ± 0.20 74.3 ± 22.3
KO (7) 3.87 ± 0.73 2.18 ± 0.61 45.4 ± 11.5
  • Extracellular glutamate levels derived from conventional microdialysis techniques revealed a similar interaction between genotype and AAV pretreatment. *p < 0.05 versus respective WT (a priori comparisons). Sample sizes are indicated in parentheses.