Pipette solution | Stimulus (cell number) | ΔCm_{net} (fF) | A_{fast} (fF) | τ_{fast} (S) | τ_{slow} (S) |
---|---|---|---|---|---|
Control | |||||
1 pulse (9) | 473 | 12.0 | |||
10 pulses at 10 Hz (11) | 1287 | 407 | 1.4 | 18.4 | |
10 pulses at 1 Hz (7) | 1607 | 217 | 1.1 | 23.0 | |
10 pulse (+75 mV) at 1 Hz (5) | 805 | 19.0 | |||
50 AP-e at 100 Hz (7) | 710 | 16.4 | |||
200 AP-e at 100 Hz (6) | 1362 | 337 | 1.9 | 19.6 | |
5 × 50 AP-e (100 Hz) at 1 Hz (8) | 1344 | 284 | 1.4 | 18.8 | |
5 × 50 AP-e (30 Hz) at 1 Hz (8) | 735 | 136 | 1.3 | 18.0 | |
10 mm EGTA | |||||
10 pulses at 1 Hz (4) | 850 | 14.3 | |||
200 AP-e at 100 Hz (6) | 756 | 16.3 |
After stimulation, the capacitance jump (ΔCm_{net}) decayed with either a monoexponential or a biexponential function as judged by eyes. The pulse refers to a 20 ms depolarization by 90 mV. The monoexponential decay was fit with the equation A(t) = ΔCm_{net} × exp (t/_{slow}), and the biexponential decay was fit with the equation A(t) = A_{fast} × exp (t/_{fast}) + A_{slow} × exp (t/_{slow}), where A_{fast} and A_{slow} are the amplitude of the fast and slow component of endocytosis, respectively, and τ_{fast} and τ_{slow} are the time constants of the fast and slow component of endocytosis, respectively. Because the net capacitance increase after stimulation (ΔCm_{net}) is the sum of A_{fast} and A_{slow}, A_{slow} is not shown in the table. Either 0.05 mm BAPTA (Control) or 10 mm EGTA was included in the pipette. The values are the mean value.