Table 1.

Behavioral and anatomical validation

TH and Nissl cells l-Dopa administration
Scores at 0 min Scores at 30 min Scores at 60 min
Groups DAT binding (fmol/mg) TH NS TH + NS Dose (mg) P D P D P D
Control
C1 123.9 922 139 1061 0.0 0.0
C2 154.5 958 113 1071 0.0 0.0
C3 134.9 1007 107 1114 0.0 0.0
C4 144.4 935 138 1073 0.0 0.0
C5 129.4 986 112 1098 0.0 0.0
MPTP
M1 1.6 137 216 353 8.0 0.0
M2 3.4 140 290 430 9.0 0.0
M3 2.7 74 237 311 7.5 0.0
M4 3.5 198 211 409 9.5 0.0
M5 1.5 135 244 379 8.5 0.0
MPTP/l-dopa nondyskinetic
L1 3.9 127 215 342 54 9.4 0.0 2.1 0.0 0.2 0.2
L2 2.9 237 172 409 59 10.6 0.0 2.8 0.0 0.0 0.0
L3 4.2 169 212 381 56 9.2 0.0 2.6 0.0 0.0 0.0
L4 5.3 134 270 404 50 8.4 0.0 2.2 0.0 0.0 0.4
MPTP/l-dopa dyskinetic
D1 8.6 246 234 480 60 8.8 0.0 2.0 0.0 0.7 3.1
D2 5.6 217 217 434 66 7.5 0.0 1.9 0.0 0.3 2.7
D3 7.7 106 164 270 53 7.2 0.0 1.8 0.0 0.0 3.1
D4 0.8 155 213 369 50 7.5 0.0 2.8 0.0 0.0 2.9
D5 4.1 178 237 415 55 10.2 0.0 2.1 0.0 0.2 2.8
  • Mean age, 3.05 ± 0.05 years; mean weight, 3.1 ± 0.1 kg. Extent of lesion is comparable among the three MPTP-lesioned groups both at the striatal [dopamine transporter (DAT) binding; femtomoles per milligram of equivalent tissue] and nigral [tyrosine hydroxylase (TH)-immunopositive neurons and Nissl-stained (NS) cells] levels [for methods, see Bezard et al. (2001c, 2003)]. l-Dopa dose (in milligrams) is given for each individual as well as their mean parkinsonian (P) and mean dyskinetic (D) scores before administration (0 min) and at 30 and 60 min after administration. The only dyskinetic animals displayed severe dyskinesia 60 min after l-dopa administration (Bezard et al., 2003), presenting choreic-athetoid (characterized by constant writhing and jerking motions), dystonic, and sometimes ballistic movements (large-amplitude flinging, flailing movements). At the peak of the dose (60-150 min after injection), dystonic rolling and writhing on the cage floor were common.