Table 2.

Primary phenotypes in RPTP-σ /δ double mutants

Genotype (σ · δ )ParalysisDiaphragm dystrophyaAberrant phrenic nerve projectionbDecreased number of motoneurons
E18.5E16.5–E18.5E12E13.5–E18.5E13E18.5
Pen (%)Pen (%)Pen (%)Pen (%)Penc (%)Red (%)Penc (%)Red (%)
+/+ · +/+0 (7)0 (4)0 (2)0 (5)0 (5)0 (5)0 (4)0 (4)
+/− · +/+0 (9)0 (7)0 (4)
−/− · +/+0 (5)0 (2)0 (7)0 (2)0 (2)
+/+ · +/−0 (12)0 (7)0 (2)0 (2)
+/− · +/−0 (27)0 (19)0 (1)
−/− · +/−0 (8)0 (5)100d (4)50 (4)30 (4)
+/+ · −/−0 (9)0 (5)0 (6)0 (3)0 (3)
+/− · −/−0 (10)0 (7)100d (5)0 (2)0 (2)
−/− · −/−100 (15)100 (6)0 (3)100e (7)0 (3)0 (3)100 (4)85 (4)
  • The number of animals analyzed is indicated in parentheses. Pen (%), Percentage of penetrance; Red (%), percentage of reduction compared with control animals; —, data are not available.

  • aScored using histological sections at E16.5 and dissected diaphragms at E18.5.

  • bScored using whole-mount anti-NF-stained diaphragms.

  • cScored positive if statistically significant by Student's t test (p < 0.05) against control animals.

  • dShowing less branching phenotype.

  • eShowing loss of sternocostal (intramuscular) nerves.