Table 1.

Effects of expression of EGFP, Math1, Id3, and E47 on cell fate in cochlear explants

cDNA %HC %Non-HC
EGFP (7) 50.1 ± 18.4 49.9 ± 18.4
Math1 (5) 100.0 ± 0.0* 0.0 ± 0.0*
Id3 (9) 5.5 ± 12.6* 94.5 ± 12.6*
E47 (8) 58.8 ± 16.9 41.2 ± 16.9
  • Expression vectors encoding full-length open reading frames for the genes listed were overexpressed in cochlear progenitor cells beginning on E13. Total number of explants analyzed for each vector are indicated in parentheses. Transfection was determined by expression of EGFP, and cell fates were determined using cell-specific markers and morphology as described in Results. Overexpression of Math1 produced a significant bias towards a hair cell fate, whereas overexpression of Id3 produced a specific bias away from the hair cell fate. Overexpression of E47 did not influence cell fate towards either cell type. Significance was determined by t test. *α = 0.01