Electrophysiological properties of neocortical L2/3 neurons in vivo
| Pyramidal cell somatic recording (n = 16) | Pyramidal cell dendritic recordinga (n = 3) | FS interneuron (n = 2) | Pyramidal cell microelectrode recording (n = 3) | Pyramidal cell with QX-314 (n = 4) | |
|---|---|---|---|---|---|
| Resting Vm, Down (mV) | −73.9 ± 1.4 | −78.7 ± 2.2 | −76.1 ± 6.4 | −75.3 ± 3.4 | −63.1 ± 3.3 |
| Resting Vm, Up (mV) | −58.9 ± 1.2 | −66.2 ± 1.2 | −56.8 ± 5.2 | −61.2 ± 4.8 | −47.9 ± 2.7 |
| ΔV (Up–Down) (mV) | 15.0 ± 0.9 | 12.6 ± 1.8 | 19.4 ± 1.2 | 14.1 ± 2.1 | 15.1 ± 3.4 |
| Vm SD, Up (mV) | 2.5 ± 0.1 | 3.2 ± 0.4 | 3.4 ± 0.3 | 3.2 ± 0.3 | |
| Mean dwell time, Down (ms) | 347 ± 32 | 343 ± 24 (n = 4) | |||
| Mean dwell time, Up (ms) | 457 ± 57 | 224 ± 26 (n = 4) | |||
| AP threshold (mV) | −36.4 ± 1.0 | −44.3 ± 2.7 | −34.1 ± 6.6 | −41.7 ± 3.2 | |
| AP amplitudeb (mV) | 66.5 ± 3.4 | 51.7 ± 8.2 | 32.2 ± 8.2 | 72.3 ± 3.2 | |
| AP half-width (ms) | 0.84 ± 0.06 | 0.89 ± 0.08 | 0.34 ± 0.05 | 0.66 ± 0.01 | |
| RN Down (MΩ) | 29.2 ± 2.0 | 25.7 ± 0.6 | 53.5 ± 3.1 | 22.5 ± 3.5 | 52.7 ± 4.8 |
| RN Discards (MΩ) | 31.6 ± 2.2 | 32.4 ± 3.7 | 54.0 ± 0.2 | 24.5 ± 6.8 | 56.3 ± 4.5 |
| RN Up (MΩ) | 36.6 ± 2.3 | 28.2 ± 1.3 | 57.3 ± 3.4 | 23.6 ± 4.4 | 44.6 ± 2.2 |
| τdecay Downc (ms) | 9.0 ± 0.6 | 8.5 ± 0.8 | 2.6 ± 0.9 | 8.9 ± 1.1 | 20.1 ± 1.2 |
| τdecay Discardsc (ms) | 10.6 ± 0.6 | 6.9 ± 2.5 | 2.7 ± 0.9 | 9.3 ± 2.7 | 21.6 ± 1.7 |
| τdecay Upc (ms) | 12.8 ± 1.1 | 16.5 ± 5.3 | 2.7 ± 0.9 | 9.9 ± 2.1 | 16.3 ± 3.8 |
| Anomalous rectification, cAR (MΩ/nA) | 18.7 ± 3.7 (n = 10) | 0.6 ± 6.4 (n = 6) | |||
| RN Down, from V–I curve (MΩ) | 27.5 ± 2.1 (n = 10) | 68.1 ± 4.8 (n = 6) | |||
| RN Up, from V–I curve (MΩ) | 44.1 ± 4.0 (n = 10) |