Table 1.

Primers for RT-PCR

Primer setSequenceTA°CNucleotidesPredicted sizeAccession numberReference
KCNQ3S-5′CAGCAAAGAACTCATCACCG3′60746–906160AF091247Shah et al., 2002
A-5′ ATGGTGGCCAGTGTGATCAG3′
KCNQ4S-5′ CCCTCCAAGCAGCATCTG3′5540–420380AF249748Shah et al., 2002
A-5′ TTGATTCGTCCCAGCATGTCCA3′
KCNQ5S-5′ GGAACCCAGCTGCCAACCTCAT3′60995–1101106AF202977Shah et al., 2002
A-5′ CTTTCTTGGTAGGGCTGCAG3′
erg1aS-5′TCCAGCGGCTGTACTCGGGC3′601994–2591597Z96106Emmi et al., 2000
A-5′TAGACCAAAAGTGGTCTGAGAACTC3′
erg2aS-5′AGGACCTGGATTGCTGGCATC3′602356–2756400AF016192Emmi et al., 2000
A-5′AGGTGAGGCTAGAGGTGACAGG3′
erg2aS-5′TCTCCAGTCAACACCCCGAC 3′602193–2851658AF016192Papa et al., 2003
A-5′CTCTGGAACTCTAGCTGCTT 3′
erg3aS-5′CAGAGAGCTGCCTGGGGCATC3′603216–3644428AF016191Emmi et al., 2000
A-5′GTCAAGCTCTGACGATGCATC3′
Degenerate ergS-5′TACTGCAA(C/T)GA(C/T)GCTTCT3′55
A-5′ GAGCCAAT(C/G)A(G/A)CATGAC(C/T)GT3′
erg1S-5′GTCACCCAGGTCCTGTCCTT3′581125–1902777Z96106
A-′GGAGAAGATCTTTTCTGAGTTGGTGTTG3′
erg2S-5′TCACAGCAGGCCGAGTG3′56509–1137628AF016192
A-5′GTAACCCAATCAAGGTGGTGG3′
erg3S-5′GAGAGAGTCAACCCAATATTGCC3′56606–1091485AF016191
A-5′GACATTTCGACCATTGTCTTCAC3′

  • ↵aPrimers used on utricular epithelium and ganglion. Other primers were used for single cells.

  • ↵TA, Annealing temperature; A, antisense; S, sense.