Summary of human NgR mutants: list of residues mutated to alanine
| No binding | Binding to AP-Ng-66 and AP-Aβ28 | Differential binding |
|---|---|---|
| 163 | 61 | 210 |
| 82, 179 | 92 | 256, 259, 284 |
| 133, 136 | 108 | |
| 158, 160 | 122 | |
| 182, 186 | 127 | |
| 211, 213 | 131 | |
| 232, 234 | 138 | |
| 111, 113, 114 | 139 | |
| 182, 186, 210 | 151 | |
| 111, 113, 114, 138 | 176 | |
| 182, 186, 158, 160 | 179 | |
| 189, 191, 211, 213 | 227 | |
| 211, 213, 237, 256, 259, 284 | 237 | |
| 171, 172, 175, 176, 196, 199, 220, 223, 224, 250 | 250 | |
| 67, 68, 71 | 259 | |
| 67, 68, 71, 89, 90, 92 | 108, 131 | |
| 87, 89, 133, 136 | 114, 117 | |
| Negative control | 127, 151 | |
| 127, 176 | ||
| 143, 144 | ||
| 189, 191 | ||
| 196, 199 | ||
| 202, 205 | ||
| 256, 259 | ||
| 267, 269 | ||
| 277, 279 | ||
| 114, 117, 139 | ||
| 189, 191, 237 | ||
| 189, 191, 284 | ||
| 202, 205, 227 | ||
| 202, 205, 250 | ||
| 220, 223, 224 | ||
| 237, 256, 259 | ||
| 296, 297, 300 | ||
| 171, 172, 175, 176 | ||
| 292, 296, 297, 300 | ||
| 196, 199, 220, 223, 224 | ||
| 171, 172, 175, 176, 196, 199 | ||
| 196, 199, 220, 223, 224, 250 | ||
| 108, 131, 61 | ||
| 36, 38 | ||
| 36, 38, 61 | ||
| 61, 131, 36, 38 | ||
| 63, 65 | ||
| 78, 81 | ||
| 87, 89 | ||
| 89, 90, 114, 117 | ||
| 95, 97 | ||
| 95, 97, 117, 119, 120, 188, 189 | ||
| 95, 97, 122 | ||
| Wild type |
Ala-substituted human NgR mutants were tested for their binding to AP-Aβ(1–28) and AP-Nogo-66. There are three categories, as follows: (1) NgR mutants that lose binding to both ligands, (2) mutants that maintain binding to all NgR ligands, and (3) differential-binding mutants that bind AP-Nogo-66 but not AP-Aβ(1–28).