Table 1.

Levels of synaptic proteins in synaptotagmin-2 knock-out mice

ProteinCortexCerebellumSpinal cord
Wild typeKnock-outWild typeKnock-outWild typeKnock-out
Syt 1(n = 4)100 ± 2.194.2 ± 3.8100 ± 4.8112.8 ± 7.4100 ± 7.2132.3 ± 2.9*
Syntaxin(n = 4)100 ± 3.2102.6 ± 4.5100 ± 10.6100.5 ± 10.7100 ± 6.783.6 ± 11.5
SNAP-25(n = 4)100 ± 2.2100.3 ± 2.3100 ± 9.690.0 ± 8.5100 ± 9.183.4 ± 10.4
Syb(n = 4)100 ± 5.0111.3 ± 3.9100 ± 8.292.8 ± 3.8100 ± 10.9106.9 ± 6.5
Rab 3(n = 4)100 ± 6.2101.2 ± 9.9100 ± 12.280.7 ± 7.1100 ± 7.786.0 ± 8.7
Syp 1(n = 4)100 ± 2.7105.6 ± 3.4100 ± 8.585.7 ± 3.1100 ± 5.191.1 ± 8.1
Synapsin(n = 4)100 ± 1.887.9 ± 1.0100 ± 5.991.3 ± 4.4100 ± 6.086.2 ± 6.3
PSD-95(n = 3)100 ± 2.4103.8 ± 4.2100 ± 3.197.9 ± 6.0100 ± 2.398.8 ± 0.5
Cpx(n = 3)100 ± 3.795.5 ± 10.2100 ± 4.489.3 ± 0.8100 ± 4.2103.0 ± 6.2
Munc18(n = 3)100 ± 6.298.3 ± 2.9100 ± 2.0102.4 ± 1.3100 ± 3.7109.3 ± 3.7
CASK(n = 3)100 ± 4.389.8 ± 0.3100 ± 0.6101.2 ± 3.7100 ± 8.593.3 ± 5.6
SCAMP(n = 3)100 ± 1.6100.8 ± 3.0100 ± 2.498.0 ± 7.5100 ± 2.198.3 ± 0.8
Synuclein(n = 3)100 ± 6.695.7 ± 5.6100 ± 2.795.5 ± 8.5100 ± 3.699.5 ± 14.2
CSP(n = 3)100 ± 4.6104.5 ± 0.8100 ± 1.3112.3 ± 7.8100 ± 5.193.2 ± 7.4
  • Total proteins in brain homogenates were analyzed by SDS-PAGE and Western blots. 125I-conjugated secondary antibodies were used and analyzed by a PhosphorImager. Cpx, Complexin; Syb, synaptobrevin 2; Syp, synaptophysin; Syt 1, synaptotagmin-1. The numbers of samples are listed in parentheses.

  • *p < 0.05.