Expression of selected growth factor mRNAs in netrin-1 wild-type and mutant animals detected by real-time RT-PCR
| ΔCTgene of interest—GAPDHa | ||||
|---|---|---|---|---|
| PDGF-AA | Neuregulin-1 | Neuregulin-3 | EphA2 | |
| +/+ (n = 5) | 2.72 ± 0.2 | 4.87 ± 0.19 | 5.49 ± 0.34 | 11.05 ± 0.18 |
| −/− (n = 7) | 2.73 ± 0.39 | 4.99 ± 0.45 | 5.5 ± 0.37 | 10.63 ± 0.5 |
| p valueb | 0.97 | 0.6 | 0.94 | 0.1 |
↵aΔCT is defined as the threshold cycle difference between the gene of interest and the housekeeping gene GAPDH. The data are expressed as mean ± SD.
↵bThree litters of netrin-1 animals were analyzed for the expression of genes listed above using a t test. In no cases were significant differences in the expression of the selected growth factor identified.