Quantitative analysis of α -synuclein and synaptophysin transport
| Direction | Number of moving particles | Frequency of movement (#/s) | Average velocity (μm/s) | Estimated % of moving objects | |
|---|---|---|---|---|---|
| α-Synuclein | Anterograde | 81 | 1/120 | 1.18 ± 0.08 | 7.6 |
| Retrograde | 62 | 1/157 | 0.99 ± 0.09 | 3.5 | |
| Synaptophysin | Anterograde | 56 | 1/10 | 1.40 ± 0.07 | 32.6 |
| Retrograde | 22 | 1/26 | 1.16 ± 0.08 | 13.3 |
Quantitative analysis of mRFP::α -SYN and SYP::GFP particles are shown. All data are derived from kymograph analysis, and synaptophysin movements were analyzed as described in Materials and Methods. Average velocities (when moving) are expressed as mean ± SEM. The proportion of moving objects was estimated as described in Materials and Methods.