Average soma size and active and passive membrane properties of acutely dissociated medium- and large-size DRG cells from control and diabetic animals
| Average soma diameter (μm) | Rs(MΩ) | Capacitance (pF) | Rin (MΩ) | RMP (−mV) | AP overshoot (mV) | AP width at base (ms) | AP width at 0 mV (ms) | AHP amplitude (mV) | AHP duration (ms) | ADP duration (ms); amplitude (mV) | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Control medium | 35.8 ± 0.2 | 4.0 ± 0.2 | 31.5 ± 0.5 | 545.0 ± 33.8 | 49.0 ± 1.0 | 40.6 ± 1.8 | 3.0 ± 0.2 | 1.2 ± 0.1 | 12.5 ± 0.7 | 40.3 ± 3.8 | 40.3 ± 3.8; 21.8 ± 1.0 |
| Diabetic medium | 35.0 ± 0.1*** | 3.6 ± 0.2 | 30.8 ± 0.4 | 505.0 ± 27.9 | 50.1 ± 0.8 | 44.6 ± 2.0 | 3.0 ± 0.3 | 0.9 ± 0.1 | 11.2 ± 0.5 | 36.3 ± 3.0 | 39.0 ± 2.2;23.1 ± 1.0 |
| Control large | 49.6 ± 0.3 | 4.5 ± 0.3 | 42.7 ± 1.7 | 207.0 ± 24.4 | 53.0 ± 2.9 | 41.2 ± 4.0 | 3.0 ± 0.2 | 1.0 ± 0.1 | 9.4 ± 1.0 | 50.0 ± 7.2 | |
| Diabetic large | 49.6 ± 0.3 | 4.6 ± 0.3 | 42.5 ± 1.7 | 226.5 ± 29.9 | 52.2 ± 2.7 | 31.3 ± 2.6* | 3.5 ± 0.2 | 1.3 ± 0.2 | 10.3 ± 1.2 | 54.1 ± 6.2 |
Rs, Series resistance; Rin, input resistance; RMP, resting membrane potential; AHP, afterhyperpolarization potential.
↵*p < 0.05 and
↵***p < 0.001 for comparison for a given cell size between control and diabetic groups. Average soma diameter, Rs, and capacitance were obtained from 161 control and 213 diabetic medium-size DRG cells and 66 control and 43 large-size DRG cells. Rin, RMP, AP, and afterhyperpolarization potential properties were obtained from 82 control and 93 diabetic medium-size DRG cells and from 20 control and 20 diabetic large-size DRG cells. ADP parameters (maximal amplitude and maximal width) were measured at RMP of −85 mV from cells presented on Figure 5B