Table 1.

Average soma size and active and passive membrane properties of acutely dissociated medium- and large-size DRG cells from control and diabetic animals

Average soma diameter (μm)Rs(MΩ)Capacitance (pF)Rin (MΩ)RMP (−mV)AP overshoot (mV)AP width at base (ms)AP width at 0 mV (ms)AHP amplitude (mV)AHP duration (ms)ADP duration (ms); amplitude (mV)
Control medium35.8 ± 0.24.0 ± 0.231.5 ± 0.5545.0 ± 33.849.0 ± 1.040.6 ± 1.83.0 ± 0.21.2 ± 0.112.5 ± 0.740.3 ± 3.840.3 ± 3.8; 21.8 ± 1.0
Diabetic medium35.0 ± 0.1***3.6 ± 0.230.8 ± 0.4505.0 ± 27.950.1 ± 0.844.6 ± 2.03.0 ± 0.30.9 ± 0.111.2 ± 0.536.3 ± 3.039.0 ± 2.2;23.1 ± 1.0
Control large49.6 ± 0.34.5 ± 0.342.7 ± 1.7207.0 ± 24.453.0 ± 2.941.2 ± 4.03.0 ± 0.21.0 ± 0.19.4 ± 1.050.0 ± 7.2
Diabetic large49.6 ± 0.34.6 ± 0.342.5 ± 1.7226.5 ± 29.952.2 ± 2.731.3 ± 2.6*3.5 ± 0.21.3 ± 0.210.3 ± 1.254.1 ± 6.2
  • Rs, Series resistance; Rin, input resistance; RMP, resting membrane potential; AHP, afterhyperpolarization potential.

  • *p < 0.05 and

  • ***p < 0.001 for comparison for a given cell size between control and diabetic groups. Average soma diameter, Rs, and capacitance were obtained from 161 control and 213 diabetic medium-size DRG cells and 66 control and 43 large-size DRG cells. Rin, RMP, AP, and afterhyperpolarization potential properties were obtained from 82 control and 93 diabetic medium-size DRG cells and from 20 control and 20 diabetic large-size DRG cells. ADP parameters (maximal amplitude and maximal width) were measured at RMP of −85 mV from cells presented on Figure 5B