Prolonged glutamatergic synaptic responses in lesioned cultures
| One stimulus | Five stimuli | |||||
|---|---|---|---|---|---|---|
| Unlesioned | Lesioned | Unlesioned with DNQX/AP5 | Unlesioned | Lesioned | Unlesioned with DNQX/AP5 | |
| Amplitude (mV) | 4.1 ± 1.0 | 2.7 ± 0.6 | 3.3 ± 0.9 | 16.4 ± 1.7 | 12.5 ± 2.0 | 14.9 ± 2.4 |
| Duration (ms) | 103 ± 25 | 103 ± 15 | 91 ± 23 | 172 ± 47 | 723 ± 335* | 378 ± 130** |
Amplitude and duration at half-maximal amplitude for responses recorded from CA1 pyramidal cells in response to local synaptic stimulation in control, unlesioned cultures (n = 7 cells), cultures lesioned 7 d earlier (n = 8 cells), and unlesioned cultures pretreated with DNQX and AP5 for 7 d (n = 6 cells). *Significantly longer than the response to five stimuli at 50 or 100 Hz in unlesioned cultures (p < 0.01; Mann–Whitney U test). **Significantly longer than the response to five stimuli at 50–100 Hz in unlesioned cultures (p < 0.05; Mann–Whitney U test). Other values are not significantly different from unlesioned cultures (Mann–Whitney U test). Cells were held at −70 mV using CsF-filled patch pipettes.