Table 1.

Colocalization (defined as percentage of EGFP-positive cells expressing ChAT) and labeling (defined as percentage of ChAT-IR cells expressing EGFP) in different brain areas

Brain region% colocalization% labeling
Nucleus basalis96 (n = 125)65 (n = 237)
Medial septum100 (n = 65)48.6 (n = 70)
Striatum97 (n = 67)35 (n = 150)
Cortex74 (n = 500)42 (n = 677)
Cranial nerve nuclei100 (n = 110)100 (n = 100)
Pedunculopontine tegmental nucleus100 (n = 46)77 (n = 47)
Laterodorsal tegmental nucleus100 (n = 100)48 (n = 188)
Parabigeminal nucleus100 (n = 300)100 (n = 150)
Spinal cord motor neurons100 (n = 196)100 (n = 211)
Spinal cord sympathetic neurons100 (n = 192)100 (n = 187)
Spinal cord central canal100 (n = 146)80 (n = 180)
Retina12 (n = 170)<1%