Table 1.

Primers for quantitative real-time RT-PCR

Gene (protein)Primer sequence: forward: reverseGenBank accession #
Aldoa (aldolase A)5′-CAA CGG TCA CAG CAC TTC G-3′NM_007438
5′-GGC TCG ACC ATA GGA GAA AG-3′
Car9 (carboanhydrase 9)5′-CGA TTG AGG CTT CCT TCC C-3′NM_139305
5′-CTA ACT CTA TCT TTG GTC CCA CTT C-3′
Epo (erythropoietin)5′-GAA AAT GTC ACG ATG GGT TGT-3′NM_007942
5′-TGT TCT TCC ACC TCC ATT CTT T-3′
EpoR (erythropoietin receptor)5′-CTC ATT CTG GTC CTC ATC TCG-3′NM_010149
5′-CAC CAC AGA CAA CCA TCA CG-3′
Gapdh (glycerin-aldehyde-3-phosphatase)5′-AGG TTG TCT CCT GCG ACT TCA-3′NM_001001303
5′-GGT GGT CCA GGG TTT CTT ACT C-3′
Hk1 (hexokinase 1)5′-GCC ATT GAA ACG GAT AAG GAA-3′NM_010438
5′-GGC TGA TCG GAA GGA GAC G-3′
Hmbs (hydroxymethylbilane synthase)5′-TCG GGG AAA CCT CAA CAC C-3′NM_013551
5′-CCT GGC CCA CAG CAT ACA T-3′
Ldha (lactate dehydrogenase A)5′-GCG GTT CCG TTA CCT GAT G-3′NM_010699
5′-GAA CCT CCT TCC ACT GCT CC-3′
Slc2a1 (glucose transporter 1)5′-GCT TCC TGC TCA TCA ATC GTA A-3′NM_011400
5′-CGA CCC TCT TCT TTC ATC TCC T-3′
Slc2a3 (glucose transporter 3)5′-TCA ACC GCT TTG GCA GAC-3′NM_011401
5′-CGA AGG GCA GTG GGA GAC-3′
Vegf (vascular endothelial growth factor)5′-GCT ACT GCC GTC CGA TTG-3′NM_001025257
5′-CTG CAG GGC TTC ATC GTT AC-3′
VEGFR1 = Flk1 (FMS-like tyrosine kinase 1)5′-GAC ATT ACC TGG ATT CTG CTA CG-3′NM_010228
5′-AAG GAT GTC TTC CCC TGT GTA 5-3′
VEGFR2 = Flk1 (fetal liver kinase 1)5′-GGA CCT GGC AGC ACG AA-3′NM_010612
5′-CAC TTC AAA GGG AGT CGG G-3′
VEGFR3 = Flt4 (FMS-like tyrosine kinase 4)5′-ACG CTG ATG ATA GTC CAC CC-3′NM_008029
5′-CGC TGT CTG TCT GGT TAT CC-3′