Table 2.

Gene expression profile of HIF-1α-regulated genes in NPCs grown under 3% oxygen conditiona

Genes (protein)HIF-1α CKO mNPCscre/wt mNPCsHIF-1α CKO 1NPCscre/wt fNPCsp valueb
Aldoa (aldolase A)5.66 ± 0.666.94 ± 2.026.88 ± 1.319.45 ± 0.760.16
Car9 (carboanhydrase 9)0.01 ± 0.000.02 ± 0.010.06 ± 0.020.14 ± 0.090.10
Epo (erythropoietin)n.d.n.d.n.d.n.d.
EpoR (erythropoietin receptor)0.07 ± 0.010.06 ± 0.010.06 ± 0.020.09 ± 0.020.69
Gapdh (glycerin-aldehyde-3-phosphatase)93.1 ± 24.7166.2 ± 34.6173.1 ± 43.0278.6 ± 21.90.24
Hk1 (hexokinase 1)2.71 ± 0.231.96 ± 0.351.71 ± 0.242.56 ± 0.200.23
Ldha (lactat deydrogenase A)26.0 ± 2.5474.4 ± 21.331.3 ± 2.2957.6 ± 6.790.18
Slc2a1 (glucose transporter 1)0.65 ± 0.051.24 ± 0.610.92 ± 0.431.92 ± 0.880.38
Slc2a3 (glucose transporter 3)0.16 ± 0.01*0.38 ± 0.060.07 ± 0.02*++0.17 ± 0.04++0.00015
Vegf (vascular endothelial growth factor)8.85 ± 2.42*18.7 ± 8.913.7 ± 1.4+18.4 ± 6.10.036
VEGFR1/Flt1 (VEGF receptor 1/FMS-like tyrosine kinase 1)0.21 ± 0.110.19 ± 0.070.04 ± 0.03+0.07 ± 0.02+0.028
VEGFR2/Flk1 (VEGF receptor 2/fetal liver kinase 1)0.30 ± 0.06*0.11 ± 0.060.0095 ± 0.001**++0.0025 ± 0.001++0.0008
VEGFR3/Flt4 (VEGF receptor 3/FMD-like tyrosine kinase 4)0.07 ± 0.030.11 ± 0.090.0029 ± 0.0010.0039 ± 0.0010.19
  • n.d., Not detectable.

  • a mRNA levels of the target gene relative to the housekeeping gene Hmbs (mean ± SEM) from three to seven experiments.

  • bp values from ANOVA tests for multiple group comparisons (n = 3–7); post hoc t test revealed statistical significant differences with *p < 0.05 and **p < 0.01 when compared with control NPCs (cre/wt mNPCs or cre/wt fNPCs); +p < 0.05 and ++p < 0.01 when compared with the respective mNPC subtype.