Table 1.

α 4YFP intensities in ventral midbrain neurons

SalineNicotineNicotine, % of salinep valueCell-based measurements (no. of cells)
Saline (6 mice)Nicotine (6 mice)
Totalα4+Totalα4+
VTA (TH+)826 ± 22902 ± 23109 ± 3NS640610655648
VTA (GAD+)268 ± 25363 ± 18136 ± 70.002736710099
SNC (TH+)1052 ± 231117 ± 25106 ± 2NS612610581579
SNR (GAD+)503 ± 17735 ± 18146 ± 4<0.001256254237237
  • Measurements of α4YFP fluorescence in various brain regions and changes with chronic nicotine (2 mg · kg−1 · h−1 for 10 d). Procedures were applied to each of the six mice in each group. Unless otherwise stated, analyses were performed on one brain section per mouse. In VTA, all TH+ or GAD+ somata were measured. In SNC, TH+ or GAD+ cell bodies were measured in one of two lobes. NS, Not significant; no., number.