Modulatory effects of coexpression of either γ2 or γ4 on glutamate-evoked (3 mm) peak amplitudes, desensitization properties, and the kainate efficacy of AMPA receptors
| n | IGlu peak (pA) | τdes (ms) | Extent of desensitization (%) | Apparent kainate efficacy | |
|---|---|---|---|---|---|
| GluR1 | |||||
| Control | 9–20 | 210.1 ± 55.8 | 6.24 ± 0.43 | 95.6 ± 1.2 | 0.04 ± 0.01 |
| +γ2 | 9–30 | 745.5 ± 141.7 | 8.55 ± 0.31 | 92.6 ± 0.8 | 0.84 ± 0.08 |
| +γ4 | 7–22 | 925.8 ± 160.2 | 19.68 ± 1.33 | 66.1 ± 4.7 | 0.75 ± 0.20 |
| GluR2(Q) | |||||
| Control | 7–8 | 249.8 ± 34.9 | 11.44 ± 0.78 | 86.1 ± 2.5 | 0.04 ± 0.01 |
| +γ2 | 6–17 | 625.8 ± 90.8 | 16.84 ± 0.92 | 57.4 ± 3.7 | 0.60 ± 0.07 |
| +γ4 | 8–14 | 468.0 ± 90.5 | 28.44 ± 3.10 | 36.4 ± 5.5 | 0.49 ± 0.11 |
| GluR3 | |||||
| Control | 5–7 | 47.2 ± 6.6 | 6.20 ± 0.75 | 98.6 ± 1.3 | 0.03 ± 0.01 |
| +γ2 | 6–8 | 108.5 ± 24.6 | 11.20 ± 1.10 | 87.9 ± 3.2 | 0.92 ± 0.12 |
| +γ4 | 4–5 | 93.0 ± 28.9 | 32.88 ± 2.92 | 43.8 ± 6.0 | 0.77 ± 0.06 |
| GluR4 | |||||
| Control | 8–10 | 458.5 ± 188.0 | 7.98 ± 0.56 | 89.5 ± 1.9 | 0.04 ± 0.01 |
| +γ2 | 5–10 | 873.2 ± 213.7 | 14.58 ± 1.32 | 78.5 ± 4.1 | 0.80 ± 0.06 |
| +γ4 | 9–12 | 658.0 ± 149.0 | 28.67 ± 2.67 | 50.4 ± 7.2 | 0.69 ± 0.06 |
| GluR1/GluR2(R) | |||||
| Control | 8–10 | 250.0 ± 20.4 | 11.42 ± 0.63 | 86.0 ± 3.5 | 0.15 ± 0.04 |
| +γ2 | 6 | 276.5 ± 32.0 | 18.70 ± 1.53 | 62.2 ± 4.8 | 0.80 ± 0.05 |
| +γ4 | 5 | 390.5 ± 60.7 | 33.38 ± 1.53 | 32.3 ± 4.2 | 0.77 ± 0.12 |
Apparent kainate efficacy: (IKA/IGlu+CTZ) with KA at 600 μ m, Glu at 3 mm, and CTZ at 300 μ m. Extent of desensitization (%): (1 − IGlu steady-state/IGlu peak) × 100, with Glu at 3 mm. Control, Without TARP.