Table 2.

GLT1+ astrocyte-enriched genes

Gene symbolGene titleEntrez geneFDCorr. p value
Known astrocyte-specific genes
    Aqp4Aquaporin 411829720.00006
    Gja1Gap junction membrane channel protein α1 (Connexin 30)1460990.00009
    Gjb6Gap junction membrane channel protein β6 (Connexin 43)14623220.00002
    S100bS100 protein, β polypeptide, neural20203320.00001
    Slc1a2Solute carrier family 1 (glial high affinity glutamate transporter), member 2205111590.00022
Cellular metabolism
    CbsCystathionine β-synthase124113610.00001
    Ppp1r3cProtein phosphatase 1, regulatory subunit 3C (PTG)5341290.00076
    Plcd4Phospholipase C, δ418802840.00018
    Slc14a1Solute carrier family 14 (urea transporter), member 1108052390.00036
    Slc4a4Solute carrier family 4 (anion exchanger), member 454403880.00003
    Slc6a11Solute carrier family 6 (GABA transporter), member 11 (GAT4)243616190.00007
Establishment of localization
    Mlc1Megalencephalic leukoencephalopathy 1170790750.00012
    TtpaTocopherol (α) transfer protein50500650.00011
    EdnrbEndothelin receptor type B13618290.00002
Nervous system development
    Hes5Hairy and enhancer of split 515208190.00009
    Fgfr3Fibroblast growth factor receptor 314184230.00002
    Tagln3Transgelin 35637070.00001
Positive regulation of cellular process
    Sox9SRY-box containing gene 920682170.00003
    Tlr3Toll-like receptor 314298030.00337
    EgfrEpidermal growth factor receptor13649520.00004
  • From an initial set of genes (19,369 probe sets), the GLT1+/Gfap-GFP+ astrocytic profile was compared with the Thy1-YFP+ neuronal profile and the GLT1/Gfap-GFP cell profile, respectively. The intersection (924 probe sets) of these two independent comparisons was extracted and called astrocyte-enriched genes. There was no statistical significant difference in any of the astrocyte-enriched genes between the GLT1+/Gfap-GFP+ and the GLT1+/Gfap-GFP astrocytic profiles (nonpaired Student's t test; Benjamini-Hochberg FDR correction of 5%). FD, Fold difference between GLT1+/Gfap-GFP−/+ astrocytes (n = 6) versus GLT1/Gfap-GFP cells (n = 3); Corr. p value, Nonpaired Student's t test, Benjamini-Hochberg FDR correction of 5%.