Table 1.

Cytokine concentrations in conditioned medium of cultured human skin fibroblasts

Control(H11)Control(H34)NPC1(UCH)NPC2(81027)
IFN-βNDND8363
IL-61.41728602040
IL-8NDND15602320
IL-1727552152
TNFα0.490.490.590.66
TGFβND0.080.040.15
G-CSF15141636
GM-CSF2.8ND4.47.5
M-CSF121212411977
  • Medium was collected from cells cultured in serum-free DMEM for 24 h and filtered through 0.22 mm pore filters. Concentrations of each cytokine were determined by ELISA. Units are picograms/milliliter. Shown are the mean values obtained in a single determination performed in duplicate. Similar results were obtained in another independent experiment. The following cytokines were at undetectable levels in all of the four cell lines: IFN-α, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-11, IL-12, IL-13, IL-18, and leptin. Soluble IL-6 receptor was also undetectable. G-CSF, Granulocyte colony-stimulating factor; GM-CSF, granulocyte–macrophage colony-stimulating factor; M-CSF, macrophage colony-stimulating factor; ND, not detectable.