Table 2.

Effect of water deprivation on the distribution of cells expressing Atf4, Cebpb, Creb3l1, Giot1, and Giot2β in the SON and PVN of the control (C) and dehydrated (D) rat hypothalamus

Atf4CebpbCreb3l1Giot1Giot2β
CDCDCDCDCD
Cells
    PVN (magnocellular)43 ± 459 ± 3*19 ± 225 ± 620 ± 253 ± 3**13 ± 251 ± 2***41 ± 260 ± 5**
    PVN (parvocellular)21 ± 232 ± 711 ± 112 ± 116 ± 217 ± 211 ± 116 ± 244 ± 350 ± 2
    SON41 ± 274 ± 3***13 ± 320 ± 228 ± 242 ± 3**13 ± 249 ± 3***37 ± 259 ± 7*
Grains/cell
    PVN (magnocellular)36 ± 393 ± 10**34 ± 331 ± 231 ± 276 ± 3**83 ± 11196 ± 13**83 ± 8185 ± 10***
    PVN (parvocellular)30 ± 260 ± 8*28 ± 231 ± 531 ± 570 ± 10*38 ± 339 ± 747 ± 499 ± 6***
    SON44 ± 4144 ± 10*34 ± 245 ± 3*28 ± 340 ± 547 ± 6219 ± 9***85 ± 6204 ± 10***
  • Cells in the SON, the magnocellular PVN, and the parvocellular PVN were quantified at 200× magnification while the numbers of silver grains were quantified at 400× magnification.

  • *p< 0.05

  • **p < 0.01

  • ***p < 0.001 (unpaired Student's t test). Data are expressed as mean ± SEM. n = 3–4 per group.